Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

• Published in: Science China. Life sciences Vol. 56; no. 1; pp. 82 - 89
• Main Authors: Xia, Bing, Dong, Chen, Zhang, WenYi, Lu, Ye, Chen, JinHui, Shi, JiSen
• Format: Journal Article
• Language: English
• Published: Beijing Science China Press 2013; Springer Nature B.V
• Subjects: Biocompatible Materials - metabolism; Biocompatible Materials - toxicity; Biological Transport; Biomedical and Life Sciences; Cell Survival - drug effects; Cells, Cultured; Fluorescein; Fluorescein - metabolism; Fluorescein-5-isothiocyanate - metabolism; Indexing in process; Life Sciences; Liriodendron - cytology; Liriodendron - embryology; Liriodendron - physiology; Microscopy, Confocal; Microscopy, Electron, Scanning; Nanomaterials; Nanoparticles; Nanoparticles - toxicity; Nanoparticles - ultrastructure; Nanostructure; Plant Cells - drug effects; Plant Cells - metabolism; Plant Cells - ultrastructure; Plant Somatic Embryogenesis Techniques - methods; Plants (organisms); Regeneration; Research Paper; Scanning electron microscopy; Silicon dioxide; Silicon Dioxide - metabolism; Silicones - metabolism; Time Factors; Viability; 二氧化硅纳米粒子; 介孔; 异硫氰酸荧光素; 悬浮细胞; 杂种鹅掌楸; 激光扫描共聚焦显微镜; 生物相容性; 高效吸收; mesoporous silica nanoparticles; endocytosis; somatic embryogenesis; cytotoxicity
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-012-4422-8

The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles （MSNs） and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope （LSCM） and scanning elec- tron microscopy （SEM）. Using fluorescein isothiocyanate （FITC） labeling, the LSCM observations demonstrated that MSNs （size, 5-15 nm） with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells, The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL-t of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regen- eration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of ex- tracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells.