Distribution of immunoreactivity for the adrenomedullin binding protein, complement factor H, in the rat brain

• Published in: Neuroscience Vol. 116; no. 4; pp. 947 - 962
• Main Authors: Serrano, J, Encinas, J.M, Fernández, A.P, Castro-Blanco, S, Alonso, D, Fernández-Vizarra, P, Richart, A, Bentura, M.L, Santacana, M, Cuttitta, F, Martı́nez, A, Rodrigo, J
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.2003; Elsevier
• Subjects: Adrenomedullin; Anatomy; Animals; Biological and medical sciences; Brain - cytology; Brain - metabolism; Brain Chemistry - physiology; Central nervous system; Complement Factor H - analysis; Complement Factor H - biosynthesis; Complement Factor H - metabolism; confocal microscopy; electron microscopy; Fundamental and applied biological sciences. Psychology; immune surveillance; Immunochemistry; immunohistochemistry; Male; Peptides - analysis; Peptides - metabolism; Protein Binding - physiology; Rats; Rats, Wistar; Vertebrates: nervous system and sense organs; PBS; ABC; FH; SDS; DAB; IR; mRNA; AM; GFAP; PB; RAMP; confocal microscopy; electron microscopy; immunohistochemistry; immune surveillance; Rat; Rodentia; Central nervous system; Adrenomedullin; Binding protein; Vertebrata; Mammalia; Distribution; Brain (vertebrata)
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/S0306-4522(02)00773-X

Adrenomedullin is a multifunctional amidated peptide that has been found in most nuclei of the CNS, where it plays a neuromodulatory role. An adrenomedullin binding protein has recently been found in plasma and characterized as complement factor H. This regulator of the complement system inhibits the progression of the complement cascade and modulates the function of adrenomedullin. Our study shows the ample distribution of factor H immunoreactivity in neurons of telencephalon, diencephalon, mesencephalon, pons, medulla, and cerebellum in the rat CNS, using immunohistochemical techniques for both light and electron microscopy. Factor H immunoreactivity was found in the cytoplasm, but nuclear staining was also a common finding. Some blood vessels and glial cells were also immunoreactive for factor H. Colocalization studies by double immunofluorescence followed by confocal microscopy revealed frequent coexistence of factor H and adrenomedullin immunoreactivities, thus providing morphological evidence for the potential interaction of these molecules in the CNS. The presence of factor H immunoreactivity in glial cells was confirmed by colocalization with glial fibrillary acidic protein. In summary, factor H is highly expressed in the CNS where it could play important roles in regulating adrenomedullin actions and contributing to an intracerebral complement system.