Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 5; pp. 1265 - 1268
• Main Authors: Makita, H.(Yokohama National Univ. (Japan)), Nakahara, Y, Fukui, H, Miyanoiri, Y, Katahira, M, Seki, H, Takeda, M, Koizumi, J
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid; Biological and medical sciences; Chromatography, High Pressure Liquid; Cysteine - analogs & derivatives; Cysteine - analysis; Cysteine - chemistry; ESPECTROMETRIA; Fundamental and applied biological sciences. Psychology; Hexuronic Acids - analysis; LEPTOTHRIUM; Leptothrix - chemistry; Leptothrix cholodnii; Magnetic Resonance Spectroscopy; Oxadiazoles - chemistry; Polysaccharides, Bacterial - chemistry; PSEUDOMONACEAE; sheath; SPECTROMETRIE; SPECTROMETRY; sheath; Residue; Bacteria; Identification; Leptothrix; 2-(cysteinyl)amido-2- deoxy-D-galacturonic acid; Leptothrix cholodnii; Galacturonic acid
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70.1265

The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fiuoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.