Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
In plants, leaf is crucial for photosynthesis and respiration. Leaf area and quantity are important for leaf vegetables to increase biomass. The process of leaf development involves coordinated regulation among small RNAs, transcription factors and hormones. Here, we found leaf size were regulated b...
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Published in | Scientific reports Vol. 7; no. 1; pp. 1395 - 9 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
03.05.2017
Nature Publishing Group Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | In plants, leaf is crucial for photosynthesis and respiration. Leaf area and quantity are important for leaf vegetables to increase biomass. The process of leaf development involves coordinated regulation among small RNAs, transcription factors and hormones. Here, we found leaf size were regulated by transcription factors NF-YA2 and NF-YA10 in
Arabidopsis
.
NF-YA2
and
NF-YA10
overexpression increased biomass accumulation through promoting leaf growth and cell expansion.
NF-YA2
and
NF-YA10
were expressed in SAM and leaf vasculature. Endogenous IAA content reduced by 20% and 24% in transgenic
Arabidopsis
plants overexpressing
NF-YA2
and
NF-YA10
compared to wild-type plants. Chromatin immunoprecipitation assays revealed that NF-YA2 and NF-YA10 bound directly to the
cis
-element CCAAT in the promoter of the
YUC2
, and decreased the expression of
YUC2
, a
YUCCA
family gene. The auxin transporter gene
PIN1
and auxin response factor1 and 2 (
ARF1
and
ARF2
) genes, transcriptional repressors, were downregulated. These findings showed leaf development was regulated by NF-YA2 and NF-YA10 through the auxin-signaling pathway and may provide a new insight into the genetic engineering of vegetables biomass and crop productivity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-01475-z |