Microbiomes of an oyster are shaped by metabolism and environment

• Published in: Scientific reports Vol. 11; no. 1; pp. 21112 - 7
• Main Authors: Scanes, Elliot, Parker, Laura M., Seymour, Justin R., Siboni, Nachshon, Dove, Michael C., O’Connor, Wayne A., Ross, Pauline M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.10.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158/2165; 631/158/2455; 631/158/855; Animals; Bacteria; Bacteria - classification; Bacteria - genetics; Carbon dioxide; Climate change; Community composition; Genotypes; Hemolymph; Hemolymph - microbiology; High temperature; Humanities and Social Sciences; Invertebrates; Marine ecosystems; Marine invertebrates; Metabolic rate; Metabolism; Microbiomes; Microbiota; multidisciplinary; Ostreidae - metabolism; Ostreidae - microbiology; Oysters; pH effects; Relative abundance; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA; Science; Science (multidisciplinary); Shellfish; Spirochetes
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-00590-2

Microbiomes can both influence and be influenced by metabolism, but this relationship remains unexplored for invertebrates. We examined the relationship between microbiome and metabolism in response to climate change using oysters as a model marine invertebrate. Oysters form economies and ecosystems across the globe, yet are vulnerable to climate change. Nine genetic lineages of the oyster Saccostrea glomerata were exposed to ambient and elevated temperature and PCO 2 treatments. The metabolic rate (MR) and metabolic by-products of extracellular pH and CO 2 were measured. The oyster-associated bacterial community in haemolymph was characterised using 16 s rRNA gene sequencing. We found a significant negative relationship between MR and bacterial richness. Bacterial community composition was also significantly influenced by MR, extracellular CO 2 and extracellular pH. The effects of extracellular CO 2 depended on genotype, and the effects of extracellular pH depended on CO 2 and temperature treatments. Changes in MR aligned with a shift in the relative abundance of 152 Amplicon Sequencing Variants (ASVs), with 113 negatively correlated with MR. Some spirochaete ASVs showed positive relationships with MR. We have identified a clear relationship between host metabolism and the microbiome in oysters. Altering this relationship will likely have consequences for the 12 billion USD oyster economy.