Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

• Published in: Scientific reports Vol. 12; no. 1; p. 2141
• Main Authors: Shakeel, Muhammad, Du, Juan, Li, Shang-Wei, Zhou, Yuan-Jin, Sarwar, Naeem, Guo, Xiaolan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.02.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/337; 631/449; 631/601; Acetyltransferase; Animals; Biosynthesis; Cnaphalocrocis medinalis; Eggs; Female; Gene expression; Gene silencing; Glucosamine; Glucosamine 6-Phosphate N-Acetyltransferase - genetics; Humanities and Social Sciences; Insect Control - methods; Insects; Male; Mortality; Moths - genetics; multidisciplinary; N-Acetyltransferase; Ovulation; Pest resistance; Pests; Phenotype; Phenotypes; Reproduction; Rice; RNA Interference; RNA-mediated interference; Science; Science (multidisciplinary); Sex ratio
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06193-9

Parental RNAi (pRNAi) is a response of RNA interference in which treated insect pests progenies showed a gene silencing phenotypes. pRNAi of CmGNA gene has been studied in Cnaphalocrocis medinalis via injection. Our results showed significant reduction in ovulation per female that was 26% and 35.26% in G1 and G2 generations, respectively. Significant reduction of hatched eggs per female were observed 23.53% and 45.26% as compared to control in G1–G2 generations, respectively. We also observed the significant variation in the sex ratio between female (40% and 53%) in G1–G2 generations, and in male (65%) in G1 generation as compared to control. Our results also demonstrated the significant larval mortality (63% and 55%) and pupal mortality (55% and 41%), and significant reduction of mRNA expression level in G1 and G2 generations. Our findings have confirmed that effectiveness of pRNAi induced silencing on the CmGNA target gene in G1–G2 generations of C. medinalis . These results suggested the potential role of pRNAi in insect pest resistance management strategies.