Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch

• Published in: Nature communications Vol. 10; no. 1; pp. 1703 - 18
• Main Authors: Tao, Hirotaka, Zhu, Min, Lau, Kimberly, Whitley, Owen K. W., Samani, Mohammad, Xiao, Xiao, Chen, Xiao Xiao, Hahn, Noah A., Liu, Weifan, Valencia, Megan, Wu, Min, Wang, Xian, Fenelon, Kelli D., Pasiliao, Clarissa C., Hu, Di, Wu, Jinchun, Spring, Shoshana, Ferguson, James, Karuna, Edith P., Henkelman, R. Mark, Dunn, Alexander, Huang, Huaxiong, Ho, Hsin-Yi Henry, Atit, Radhika, Goyal, Sidhartha, Sun, Yu, Hopyan, Sevan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.04.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 147/3; 38/70; 42/41; 631/136/1660; 631/80/79/2066; 64/60; 82/51; 9/10; Actin Cytoskeleton; Actomyosin; Actomyosin - metabolism; Animals; Arches; Calcium; Calcium - metabolism; Cell Cycle; Cell Polarity; Cortex; Cytoskeleton; Cytoskeleton - physiology; Cytosol - metabolism; Elasticity; Embryos; Epithelial Cells - metabolism; Genetic code; Genomes; Green Fluorescent Proteins - metabolism; Humanities and Social Sciences; Mandible; Mandible - embryology; Mandible - physiology; Mesenchyme; Mice; multidisciplinary; Mutation; Oscillometry; Polarity; Primordia; Rigidity; Science; Science (multidisciplinary); Signal Transduction; Stress, Mechanical; Vertebrates; Vinculin; Vinculin - metabolism; Viscosity; Wnt protein; Wnt-5a Protein - physiology; Yes-associated protein; Zebrafish
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-09540-z

Multiple vertebrate embryonic structures such as organ primordia are composed of confluent cells. Although mechanisms that shape tissue sheets are increasingly understood, those which shape a volume of cells remain obscure. Here we show that 3D mesenchymal cell intercalations are essential to shape the mandibular arch of the mouse embryo. Using a genetically encoded vinculin tension sensor that we knock-in to the mouse genome, we show that cortical force oscillations promote these intercalations. Genetic loss- and gain-of-function approaches show that Wnt5a functions as a spatial cue to coordinate cell polarity and cytoskeletal oscillation. These processes diminish tissue rigidity and help cells to overcome the energy barrier to intercalation. YAP/TAZ and PIEZO1 serve as downstream effectors of Wnt5a -mediated actomyosin polarity and cytosolic calcium transients that orient and drive mesenchymal cell intercalations. These findings advance our understanding of how developmental pathways regulate biophysical properties and forces to shape a solid organ primordium. Morphogenesis of tissue sheets is well studied, but mechanisms that shape bulk tissues are unclear. Here, the authors show that mesenchymal cells intercalate in 3D to shape the mouse branchial arch, with cortical forces driving intercalations in a Wnt5a-, Yap/Taz- and Piezo1-dependent manner.