Detecting disease associated biomarkers by luminescence modulating phages

• Published in: Scientific reports Vol. 12; no. 1; p. 2433
• Main Authors: Kulpakko, Janne, Juusti, Vilhelmiina, Rannikko, Antti, Hänninen, Pekka E.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.02.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/45; 631/57; 692/4017; Bacteriophages - metabolism; Biomarkers; Biomarkers, Tumor - urine; Biosensing Techniques - methods; C-reactive protein; C-Reactive Protein - urine; Disease; Dyes; Humanities and Social Sciences; Humans; Inflammation; Kallikreins - urine; Luminescence; Luminescent Measurements - methods; Male; multidisciplinary; Neoplasm Grading; Phages; Prospective Studies; Prostate cancer; Prostate-Specific Antigen - urine; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - urine; Registries; Retrospective Studies; Risk assessment; Science; Science (multidisciplinary); Sensitivity and Specificity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06433-y

Assessment of risk for a given disease and the diagnosis of diseases is often based on assays detecting biomarkers. Antibody-based biomarker-assays for diseases such as prostate cancer are often ambiguous and biomarker proteins are frequently also elevated for reasons that are unspecific. We have opted to use luminescence modulating phages for the analysis of known acute inflammatory response biomarker CRP (C-reactive protein) and biomarkers of prostate cancer in urine samples. Firstly, CRP was used to simulate the detection process in a controlled chemical environment. Secondly, we tried to classify more challenging lethal prostate cancer samples from control samples. Our unique method utilizes a special biopanning process in order to create special phages capable of capturing a dye necessary for detection and potential biomarkers. As the biomarker-molecules interfere with the phages, dye is repelled from the phage network resulting in an altered reporter luminescence. These changes can be observed with an absorbance reader and even with the naked eye. The simple method could present an alternative for screening of disease biomarkers. For prostate cancer urine samples, we achieved a sensitivity of 80% and specificity of 75% to detect Grade Group (GG) 4 and 5 prostate cancer.