Fabrication of Three-Dimensional Cell Constructs Using Temperature-Responsive Hydrogel

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3...

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Published inTissue engineering. Part A Vol. 16; no. 8; pp. 2497 - 2504
Main Authors Sasaki, Jun-Ichi, Asoh, Taka-Aki, Matsumoto, Takuya, Egusa, Hiroshi, Sohmura, Taiji, Alsberg, Eben, Akashi, Mitsuru, Yatani, Hirofumi
Format Journal Article
LanguageEnglish
Published United States Mary Ann Liebert, Inc 01.08.2010
Subjects
Acrylamide
Acrylic Resins - chemistry
Animals
Biocompatible Materials - chemistry
Biomedical materials
Cell Culture Techniques - methods
Cell Line
Cell physiology
Cellular biology
Chemical properties
Hydrogels - chemistry
Materials science
Materials Testing
Mice
Original Articles
Osteoblasts - cytology
Osteoblasts - physiology
Polymers
Surface Properties
Technology application
Temperature
Tissue engineering
Tissue Engineering - methods
United States
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Summary:A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly- N -isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.
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ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2009.0523