PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegans

• Published in: Nature communications Vol. 9; no. 1; pp. 1195 - 17
• Main Authors: Wu, Jieyu, Jiang, Xue, Li, Yamei, Zhu, Tingting, Zhang, Jingjing, Zhang, Zhiguo, Zhang, Linqiang, Zhang, Yuru, Wang, Yanli, Zou, Xiaoju, Liang, Bin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.03.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/89; 631/443/319; 631/80/86/2366; 64; 64/11; 82/1; Accumulation; Acetyl-CoA carboxylase; Actinomycin; Biosynthesis; Deactivation; Diacylglycerol O-acyltransferase; Diglycerides; Energy balance; Energy metabolism; Energy storage; Fatty acids; Fatty-acid synthase; Gene expression; Humanities and Social Sciences; Inactivation; Lipids; Lipogenesis; Metabolism; multidisciplinary; Nucleoli; Ribonucleic acid; Ribosomal protein S6; Ribosomal protein S6 kinase; RNA; RNA processing; rRNA; Science; Science (multidisciplinary); Starvation; Stress; Stresses
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-03531-2

The primary function of the nucleolus is ribosome biogenesis, which is an extremely energetically expensive process. Failures in ribosome biogenesis cause nucleolar stress with an altered energy status. However, little is known about the underlying mechanism linking nucleolar stress to energy metabolism. Here we show that nucleolar stress is triggered by inactivation of RSKS-1 (ribosomal protein S6 kinase), RRP-8 (ribosomal RNA processing 8), and PRO-2/3 (proximal proliferation), all of which are involved in ribosomal RNA processing or inhibition of rDNA transcription by actinomycin D (AD), leading to excessive lipid accumulation in Caenorhabditis elegans . The transcription factor PHA-4/FoxA acts as a sensor of nucleolar stress to bind to and transactivate the expression of the lipogenic genes pod-2 (acetyl-CoA carboxylase), fasn-1 (fatty acid synthase), and dgat-2 (diacylglycerol O -acyltransferase 2), consequently promoting lipid accumulation. Importantly, inactivation of pha-4 or dgat-2 is sufficient to abolish nucleolar stress-induced lipid accumulation and prolonged starvation survival. The results revealed a distinct PHA-4-mediated lipogenesis pathway that senses nucleolar stress and shifts excessive energy for storage as fat. Nucleolar stress can disrupt ribosome biogenesis and in turn energy metabolism and lipid storage, but how this is regulated is unclear. Here, the authors show in C. elegans that the transcription factor PHA-4/FOXA acts as a sensor for nucleolar stress and can regulate expression of lipogenic genes