Gut microbiome in PCOS associates to serum metabolomics: a cross-sectional study

• Published in: Scientific reports Vol. 12; no. 1; p. 22184
• Main Authors: Yu, Zheng, Qin, Erqi, Cheng, Shirui, Yang, Han, Liu, Rui, Xu, Tian, Liu, Yanqin, Yuan, Jing, Yu, Shuguang, Yang, Jie, Liang, Fanrong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.12.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 692/53/2423; 692/699/2743/2730; Acids; Alistipes; Bacteroidetes - genetics; Bilirubin; Citric acid; Cross-Sectional Studies; Digestive system; Energy metabolism; Escherichia; Female; Gastrointestinal Microbiome; Humanities and Social Sciences; Humans; Insulin; Insulin resistance; Intestinal microflora; Lecithin; Lipid metabolism; Lysophosphatidylcholine; Metabolic disorders; Metabolism; Metabolites; Metabolomics; Microbiomes; Microbiota; multidisciplinary; Niacin; Phosphatidylcholine; Polycystic ovary syndrome; Polycystic Ovary Syndrome - metabolism; RNA, Ribosomal, 16S - genetics; rRNA 16S; Science; Science (multidisciplinary); Shigella
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-25041-4

The association between gut microbiome and chronic metabolic disease including polycystic ovary syndrome (PCOS), is well documented, however, the relationship between the gut microbiota and serum metabolites remains unknown. In this study, untargeted metabolomics together with a 16S rRNA gene sequencing tool was used to detect small molecule serum metabolites and the gut microbiome. We identified 15 differential metabolites between PCOS patients and the healthy control. Lysophosphatidylcholine (LPC) (18:2, 20:3, 18:1, P-16:0, 17:0, 15:0, 18:3, 20:4), phosphatidylcholine(PC), ganglioside GA2 (d18:1/16:0) and 1-linoleoylglycerophosphocholine were increased in the PCOS group, and the concentrations of phosphoniodidous acid, bilirubin, nicotinate beta- d -ribonucleotide and citric acid were decreased in the PCOS group, suggesting a lipid metabolism and energy metabolism disorder in the PCOS patients. The diversity of gut microbiota in PCOS group was lower than that in healthy controls. Escherichia/Shigella , Alistipes and an unnamed strain 0319_6G20 belonging to Proteobacteria were important distinguishing genera (LDA > 3.5) in PCOS. Prevotella_9 was positively correlated with phosphoniodidous acid, nicotinate beta- d -ribonucleotide and citric acid concentrations, and negatively correlated with the concentration of LPC (20:3) and 1-linoleoylglycerophosphocholine; Roseburia was negatively correlated with LPC concentration (20:4), while the characteristic genus 0319_6G20 of PCOS was positively correlated with LPC concentration (20:3) (COR > 0.45). SF-36 in the PCOS group was significantly lower than that in the healthy control (HC) group, which was associated with the presence of Escherichia-Shigella and Alistipes . Our finding demonstrated the correlation between the gut microbiota and serum metabolites in PCOS, and therefore characteristic gut microbiota and metabolites may play an important role in the insulin resistance and the mood changes of PCOS patients.