Dietary protein restriction throughout intrauterine and postnatal life results in potentially beneficial myocardial tissue remodeling in the adult mouse heart

• Published in: Scientific reports Vol. 9; no. 1; pp. 15126 - 17
• Main Authors: Hennig, Maria, Ewering, Lea, Pyschny, Simon, Shimoyama, Shinya, Olecka, Maja, Ewald, Dominik, Magarin, Manuela, Uebing, Anselm, Thierfelder, Ludwig, Jux, Christian, Drenckhahn, Jörg-Detlef
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.10.2019; Nature Publishing Group
• Subjects: 13/51; 38/77; 631/136/3194; 631/443/592/2726; 64/60; 692/4019/592/1540; 82/51; 82/80; 96/63; Aging - physiology; Amino Acids - deficiency; Animals; Animals, Newborn; Body Weight; Capillaries - physiology; Cardiomyocytes; Cardiovascular system; Cell Count; Cell Proliferation; Cell Size; Diet; Diet, Protein-Restricted; Dietary restrictions; Echocardiography; Feeding Behavior; Female; Females; Fibrosis; Genetic crosses; Heart; Heart - anatomy & histology; Heart - physiology; Heart Ventricles; Histology; Humanities and Social Sciences; Hypocaloric diet; Low protein diet; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Muscle contraction; Myocardium; Myocytes, Cardiac - cytology; Neonates; Neovascularization, Physiologic; Nutrient deficiency; Offspring; Organ Size; Pregnancy; Proteins; Science; Science (multidisciplinary); Sex Characteristics; Sex differences; Signal Transduction; Vascular Endothelial Growth Factor A - metabolism; Vascularization; Ventricle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-51654-3

Diet composition impacts metabolic and cardiovascular health with high caloric diets contributing to obesity related disorders. Dietary interventions such as caloric restriction exert beneficial effects in the cardiovascular system, but alteration of which specific nutrient is responsible is less clear. This study investigates the effects of a low protein diet (LPD) on morphology, tissue composition and function of the neonatal and adult mouse heart. Mice were subjected to LPD (8.8% protein) or standard protein diet (SPD, 22% protein) throughout intrauterine and postnatal life. At birth LPD female but not male offspring exhibit reduced body weight whereas heart weight was unchanged in both sexes. Cardiomyocyte cross sectional area was increased in newborn LPD females compared to SPD, whereas proliferation, cellular tissue composition and vascularization were unaffected. Adult female mice on LPD exhibit reduced body weight but normal heart weight compared to SPD controls. Echocardiography revealed normal left ventricular contractility in LPD animals. Histology showed reduced interstitial fibrosis, lower cardiomyocyte volume and elevated numbers of cardiomyocyte and non-myocyte nuclei per tissue area in adult LPD versus SPD myocardium. Furthermore, capillary density was increased in LPD hearts. In conclusion, pre- and postnatal dietary protein restriction in mice causes a potentially beneficial myocardial remodeling.