Prolonged exposure to multi-walled carbon nanotubes dysregulates intestinal mir-35 and its direct target MAB-3 in nematode Caenorhabditis elegans

• Published in: Scientific reports Vol. 9; no. 1; pp. 12144 - 9
• Main Authors: Zhao, Yunli, Jin, Ling, Wang, Yuan, Kong, Yan, Wang, Dayong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.08.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 3' Untranslated Regions; 38; 38/89; 45; 64/11; 692/53; 704/172/4081; Animals; Base Sequence; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - antagonists & inhibitors; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; DNA-Binding Proteins - antagonists & inhibitors; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Forkhead Transcription Factors - antagonists & inhibitors; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Gene Expression Regulation - drug effects; Humanities and Social Sciences; Insulin; Insulin - metabolism; Intestinal Mucosa - metabolism; Intestine; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; multidisciplinary; Nanotechnology; Nanotubes; Nanotubes, Carbon - toxicity; Nematodes; Reactive Oxygen Species - metabolism; Science; Science (multidisciplinary); Sequence Alignment; Signal transduction; Signal Transduction - drug effects; Toxicity; Worms
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-48646-8

In nematode Caenorhabditis elegans , some microRNAs (miRNAs) could be dysregulated by multi-walled carbon nanotubes (MWCNTs), suggesting their involvement in regulating the response of nematodes to MWCNTs. Among these dysregulated miRNAs induced by MWCNT exposure, prolonged exposure to MWCNTs increased mir - 35 expression. mir - 35 further acted in the intestine to regulate the response to MWCNTs. In the intestine, a transcription factor MAB-3 was identified as its target in regulating the response to MWCNTs. Moreover, during the control of response to MWCNTs, MAB-3 acted upstream of DAF-16, a fork head transcriptional factor in insulin signaling pathway. Therefore, MWCNTs exposure potentially dysregulates intestinal mir - 35 and its direct target MAB-3, which may activate a protective intestinal response of nematodes against the MWCNTs toxicity.