Impact of pre-existing dengue immunity on human antibody and memory B cell responses to Zika

• Published in: Nature communications Vol. 10; no. 1; pp. 938 - 13
• Main Authors: Andrade, Paulina, Gimblet-Ochieng, Ciara, Modirian, Faraz, Collins, Matthew, Cárdenas, Maritza, Katzelnick, Leah C., Montoya, Magelda, Michlmayr, Daniela, Kuan, Guillermina, Balmaseda, Angel, Coloma, Josefina, de Silva, Aravinda M., Harris, Eva
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.02.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 13/106; 14/34; 631/250/2152/1566; 631/250/2152/2153; 631/326/596/1413; 692/699/255/2514; Adolescent; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; B-Lymphocytes - immunology; Child; Convalescence; Cross Reactions; Cross-reactivity; Dengue - complications; Dengue - immunology; Dengue fever; Dengue Virus - classification; Dengue Virus - immunology; Female; Humanities and Social Sciences; Humans; Immunity; Immunoglobulins; Immunologic Memory; Immunological memory; Lymphocytes B; Male; multidisciplinary; Neutralizing; Science; Science (multidisciplinary); Vector-borne diseases; Viral diseases; Viruses; Zika virus; Zika Virus - immunology; Zika Virus Infection - complications; Zika Virus Infection - immunology
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-08845-3

Little is known about enduring memory B cell (MBC) responses to Zika virus (ZIKV) and their relationship with circulating antibodies. Here we comprehensively assess MBC frequency and specificity alongside serum binding and neutralizing antibody responses to ZIKV ~2 weeks and ~8 months postinfection in 31 pediatric subjects with 0, 1 or >1 prior infections with the related dengue virus (DENV). ZIKV infection elicits a robust type-specific MBC response, and the majority of late convalescent anti-ZIKV serum neutralizing activity is attributable to ZIKV-specific antibodies. The number of prior DENV infections does not influence type-specific or cross-reactive MBC responses, although ZIKV has the highest cross-reactivity with DENV3. DENV cross-reactive MBCs expanded by ZIKV infection decline in number and proportion by late convalescence. Finally, ZIKV induces greater cross-reactivity in the MBC pool than in serum antibodies. Our data suggest immunity to DENV only modestly shapes breadth and magnitude of enduring ZIKV antibody responses. Here, Andrade et al. assess the memory B cell (MBC) and antibody response to Zika virus (ZIKV) in individuals with and without prior dengue virus (DENV) infection and find that ZIKV infection elicits a robust and specific MBC response that is only modestly affected by the number of prior DENV infections.