Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host

• Published in: Communications biology Vol. 4; no. 1; p. 345
• Main Authors: Bermúdez-Méndez, Erick, Katrukha, Eugene A., Spruit, Cindy M., Kortekaas, Jeroen, Wichgers Schreur, Paul J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.03.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 14/1; 14/32; 631/326/421; 631/326/596/2557; Animals; Biology; Biomedical and Life Sciences; Chlorocebus aethiops; Coccidioidomycosis; Fluorescence in situ hybridization; Fluorescent Antibody Technique; Genomes; Genomics; Immunofluorescence; In Situ Hybridization, Fluorescence; Insect cells; Insecta - virology; Life Sciences; Mammalian cells; Offspring; Orthobunyavirus - genetics; Orthobunyavirus - metabolism; Orthobunyavirus - pathogenicity; Packaging; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; Rift Valley fever; Rift Valley fever virus - genetics; Rift Valley fever virus - metabolism; Rift Valley fever virus - pathogenicity; Segmentation; Vero Cells; Viral Genome Packaging; Viral Proteins - genetics; Viral Proteins - metabolism; Virion - genetics; Virion - metabolism; Virions
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-021-01821-y

Bunyaviruses have a genome that is divided over multiple segments. Genome segmentation complicates the generation of progeny virus, since each newly formed virus particle should preferably contain a full set of genome segments in order to disseminate efficiently within and between hosts. Here, we combine immunofluorescence and fluorescence in situ hybridization techniques to simultaneously visualize bunyavirus progeny virions and their genomic content at single-molecule resolution in the context of singly infected cells. Using Rift Valley fever virus and Schmallenberg virus as prototype tri-segmented bunyaviruses, we show that bunyavirus genome packaging is influenced by the intracellular viral genome content of individual cells, which results in greatly variable packaging efficiencies within a cell population. We further show that bunyavirus genome packaging is more efficient in insect cells compared to mammalian cells and provide new insights on the possibility that incomplete particles may contribute to bunyavirus spread as well. Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.