Clean-up method of forchlorfenuron in agricultural products for HPLC analysis

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 48; no. 5; pp. 148 - 152
• Main Authors: Kobayashi, M.(Tokyo-to. Inst. of Public Health (Japan)), Takano, I, Tamura, Y, Tomizawa, S, Tateishi, Y, Sakai, N, Kamijo, K, Ibe, A, Nagayama, T
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society for Food Hygiene and Safety 01.10.2007
• Subjects: ACETONA; ACETONE; AGRICULTURAL PRODUCTS; AGROCHEMICALS; ANALYTICAL METHODS; CAPTEUR; Chemistry Techniques, Analytical - methods; Chromatography, High Pressure Liquid; Crops, Agricultural - chemistry; EXTRACCION; EXTRACTION; forchlorfenuron; HPLC; Phenylurea Compounds - analysis; plant growth regulator; Plant Growth Regulators - analysis; PLANT GROWTH SUBSTANCES; PRODUCTOS AGRICOLAS; PRODUIT AGRICOLE; PURIFICACION; PURIFICATION; Pyridines - analysis; RADIACION ULTRAVIOLETA; RAYONNEMENT ULTRAVIOLET; SENSORES; SENSORS; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; ULTRAVIOLET RADIATION; UV detection
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.48.148

A simplified method for the determination of forchlorfenuron in agricultural products by HPLC with UV detection was investigated. A chopped sample homogenate from agricultural products was extracted with acetone. The extract was filtrated and concentrated. The residues was loaded onto a Chem Elut column and extracted with ethyl acetate. The crude extract was purified on Oasis HLB and Bond Elut PSA mini-columns using a mixture of methanol and ethyl acetate. Forchlorfenuron was analyzed by HPLC with UV detection (263 nm). HPLC separation was performed on an ODS column with methanol-water as the mobile phase. Recoveries of forchlorfenuron from several agricultural products fortified at the level of 0.1 micro g/g were in the range of 87.6-99.5%. The limit of detection (S/N=3) was 0.005 micro g/g in the sample.