Effects of seaweed extracts on in vitro rumen fermentation characteristics, methane production, and microbial abundance

• Published in: Scientific reports Vol. 11; no. 1; p. 24092
• Main Authors: Choi, Youyoung, Lee, Shin Ja, Kim, Hyun Sang, Eom, Jun Sik, Jo, Seong Uk, Guan, Le Luo, Seo, Jakyeom, Kim, Hanbeen, Lee, Sang Suk, Lee, Sung Sill
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.12.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/449; 631/601; 704/172; Abundance; Algae; Animals; Batch culture; Carbon dioxide; Carbon Dioxide - metabolism; Fatty Acids, Volatile; Fermentation; Fermentation - drug effects; Flavonoids; Gas production; Gases - metabolism; Humanities and Social Sciences; In Vitro Techniques; Incubation; Metabolites; Methane; Methane - metabolism; Methanogenic archaea; multidisciplinary; Oil and gas production; Plant Extracts - chemistry; Plant Extracts - pharmacology; Propionates; Propionic acid; Rumen; Rumen - metabolism; Rumen - microbiology; Science; Science (multidisciplinary); Seaweed - chemistry; Seaweeds; Time Factors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-03356-y

Several seaweed extracts have been reported to have potential antimethanogenic effects in ruminants. In this study, the effect of three brown seaweed species ( Undaria pinnatifida , UPIN ; Sargassum fusiforme , SFUS ; and Sargassum fulvellum , SFUL ) on rumen fermentation characteristics, total gas, methane (CH 4 ), carbon dioxide (CO 2 ) production, and microbial populations were investigated using an in vitro batch culture system. Seaweed extract and its metabolites, total flavonoid and polyphenol contents were identified and compared. For the in vitro batch, 0.25 mg∙mL −1 of each seaweed extract were used in 6, 12, 24, 36 and 48 h of incubation. Seaweed extract supplementation decreased CH 4 yield and its proportion to total gas production after 12, 24, and 48 h of incubation, while total gas production were not significantly different. Total volatile fatty acid and molar proportion of propionate increased with SFUS and SFUL supplementation after 24 h of incubation, whereas UPIN was not affected. Additionally, SFUS increased the absolute abundance of total bacteria, ciliate protozoa, fungi, methanogenic archaea, and Fibrobacter succinogenes . The relative proportions of Butyrivibrio fibrisolvens , Butyrivibrio proteoclasticus, and Prevotella ruminicola were lower with seaweed extract supplementation, whereas Anaerovibrio lipolytica increased. Thus, seaweed extracts can decrease CH 4 production, and alter the abundance of rumen microbial populations.