Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

• Published in: Nature communications Vol. 9; no. 1; pp. 594 - 12
• Main Authors: Wang, Yan, Xu, Yuanpeng, Sun, Yujing, Wang, Huibin, Qi, Jiaming, Wan, Bowen, Ye, Wenwu, Lin, Yachun, Shao, Yuanyuan, Dong, Suomeng, Tyler, Brett M., Wang, Yuanchao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.02.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 38/47; 38/77; 38/88; 38/90; 42; 42/44; 49/22; 631/1647/2163; 631/449/2169/2107; 631/449/2675; 82/1; 82/80; 82/83; Amino Acid Sequence; Apoplast; Disease resistance; Disease Resistance - genetics; Eukaryotes; Gene Expression Regulation, Plant; Genes; Genomes; Glycoside hydrolase; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Humanities and Social Sciences; Hydrolase; Immunity; Innate immunity; Kinases; Leucine; Leucine-Rich Repeat Proteins; multidisciplinary; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - microbiology; Phylogeny; Phytophthora - physiology; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - microbiology; Plant Proteins - classification; Plant Proteins - genetics; Plants, Genetically Modified; Protein Serine-Threonine Kinases - classification; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Proteins; Proteins - classification; Proteins - genetics; Proteins - metabolism; Reactive Oxygen Species - metabolism; Receptors; Science; Science (multidisciplinary); Sequence Homology, Amino Acid; Tobacco; Viruses
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-03010-8

Activation of innate immunity by membrane-localized receptors is conserved across eukaryotes. Plant genomes contain hundreds of such receptor-like genes and those encoding proteins with an extracellular leucine-rich repeat (LRR) domain represent the largest family. Here, we develop a high-throughput approach to study LRR receptor-like genes on a genome-wide scale. In total, 257 tobacco rattle virus-based constructs are generated to target 386 of the 403 identified LRR receptor-like genes in Nicotiana benthamiana for silencing. Using this toolkit, we identify the LRR receptor-like protein Response to XEG1 (RXEG1) that specifically recognizes the glycoside hydrolase 12 protein XEG1. RXEG1 associates with XEG1 via the LRR domain in the apoplast and forms a complex with the LRR receptor-like kinases BAK1 and SOBIR1 to transduce the XEG1-induced defense signal. Thus, this genome-wide silencing assay is demonstrated to be an efficient toolkit to pinpoint new immune receptors, which will contribute to developing durable disease resistance. The role of most plant leucine-rich repeat (LRR) receptors in innate immunity is unknown. Here, the authors develop virus-based constructs to silence LRR receptor-like genes in the Nicotiana benthamiana genome and identify Response to XEG1 that specifically recognizes the glycoside hydrolase 12 protein XEG1.