A Microfluidic System for Detecting Tumor Cells Based on Biomarker Hexaminolevulinate (HAL): Applications in Pleural Effusion

Malignant pleural effusion is a common clinical problem, which often occurs in cases of malignant tumors, especially in lung cancer. In this paper, a pleural effusion detection system based on a microfluidic chip, combined with specific tumor biomarker, hexaminolevulinate (HAL), used to concentrate...

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Published inMicromachines (Basel) Vol. 14; no. 4; p. 771
Main Authors Luan, Yiran, Li, Lei, Xun, Xiaoyi, Wang, Yang, Wei, Xinyue, Zheng, Yuqun, Fan, Zhijuan, Sun, Xuguo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 30.03.2023
MDPI
Subjects
Biomarkers
Cancer
Cells
Cellular biology
Detectors
Flow velocity
hexaminolevulinate
Lung cancer
Metastasis
microfluidic chip
Microfluidics
Morphology
Optimization
Pleural effusion
Pleural effusions
tumor cells
Tumors
China
Beijing China
United States > US
Japan
hexaminolevulinate
microfluidic chip
tumor cells
pleural effusion
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Summary:Malignant pleural effusion is a common clinical problem, which often occurs in cases of malignant tumors, especially in lung cancer. In this paper, a pleural effusion detection system based on a microfluidic chip, combined with specific tumor biomarker, hexaminolevulinate (HAL), used to concentrate and identify tumor cells in pleural effusion was reported. The lung adenocarcinoma cell line A549 and mesothelial cell line Met-5A were cultured as the tumor cells and non-tumor cells, respectively. The optimum enrichment effect was achieved in the microfluidic chip when the flow rates of cell suspension and phosphate-buffered saline achieved 2 mL/h and 4 mL/h, respectively. At the optimal flow rate, the proportion of A549 increased from 28.04% to 70.01% due to the concentration effect of the chip, indicating that tumor cells could be enriched by a factor of 2.5 times. In addition, HAL staining results revealed that HAL can be used to identify tumor cells and non-tumor cells in chip and clinical samples. Additionally, the tumor cells obtained from the patients diagnosed with lung cancer were confirmed to be captured in the microfluidic chip, proving the validity of the microfluidic detection system. This study preliminarily demonstrates the microfluidic system is a promising method with which to assist clinical detection in pleural effusion.
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These authors contributed equally to this work.
ISSN:2072-666X
2072-666X
DOI:10.3390/mi14040771