Ciliary neurotrophic factor is increased in the plasma of patients with obesity and its levels correlate with diabetes and inflammation indices

• Published in: Scientific reports Vol. 12; no. 1; pp. 8331 - 12
• Main Authors: Perugini, Jessica, Di Mercurio, Eleonora, Giuliani, Angelica, Sabbatinelli, Jacopo, Bonfigli, Anna Rita, Tortato, Elena, Severi, Ilenia, Cinti, Saverio, Olivieri, Fabiola, le Roux, Carel W., Gesuita, Rosaria, Giordano, Antonio
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.05.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 692/163; 692/163/2743/137; 692/163/2743/2037; 692/163/2743/393; Ciliary neurotrophic factor; Ciliary Neurotrophic Factor - pharmacology; Ciliary Neurotrophic Factor Receptor alpha Subunit; Diabetes; Diabetes Mellitus; Female; Humanities and Social Sciences; Humans; Inflammation; Insulin; Insulin resistance; Interleukin 6; Leptin; Male; multidisciplinary; Obesity; Patients; Plasma; Plasma levels; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-11942-x

To establish whether obesity involves activation of endogenous ciliary neurotrophic factor (CNTF) signalling, we evaluated its plasma levels in patients with obesity and correlated its values with the major clinical and haematological indices of obesity, insulin resistance and systemic inflammation. This study involved 118 subjects: 39 healthy controls (19 men), 39 subjects with obesity (19 men) and 40 subjects with obesity and diabetes (20 men). Plasma CNTF and CNTF receptor α (CNTFRα) were measured using commercial ELISA kits. The results showed that plasma CNTF was significantly higher in males and females with obesity with and without diabetes than in healthy subjects. Women consistently exhibited higher levels of circulating CNTF. In both genders, CNTF levels correlated significantly and positively with obesity (BMI, WHR, leptin), diabetes (fasting insulin, HOMA index and HbA1c) and inflammation (IL-6 and hsCRP) indices. Circulating CNTFRα and the CNTF/CNTFRα molar ratio tended to be higher in the patient groups than in controls. In conclusion, endogenous CNTF signalling is activated in human obesity and may help counteract some adverse effects of obesity. Studies involving a higher number of selected patients may reveal circulating CNTF and/or CNTFRα as potential novel diagnostic and/or prognostic markers of obesity, diabetes and associated diseases.