Evaluation of the antibacterial effects and mechanism of Plantaricin 149 from Lactobacillus plantarum NRIC 149 on the peri-implantitis pathogens

• Published in: Scientific reports Vol. 11; no. 1; p. 21022
• Main Authors: Lin, Xiaolong, Xu, Jiajia, Shi, Zhiwei, Xu, Yuedan, Fu, Tao, Zhang, Ling, He, Fuming
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.10.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326; 692/308; Amino Acid Sequence; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiosis; Antimicrobial resistance; Bacteriocins - chemistry; Bacteriocins - pharmacology; Biofilms; Biofilms - drug effects; Bone marrow; Cytotoxicity; Dose-Response Relationship, Drug; Fluorescence; Fluorescence microscopy; Humanities and Social Sciences; Lactobacillus plantarum; Lactobacillus plantarum - genetics; Lactobacillus plantarum - isolation & purification; Lactobacillus plantarum - metabolism; Lactobacillus plantarum - ultrastructure; Mice; Microbial Sensitivity Tests; Microbial Viability - drug effects; Microorganisms; Microscopy; multidisciplinary; Pathogens; Peptides - chemistry; Peptides - pharmacology; Peri-Implantitis - drug therapy; Peri-Implantitis - microbiology; Polymerase chain reaction; Scanning electron microscopy; Science; Science (multidisciplinary); Supplements; Teeth
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-00497-y

Peri-implantitis is a common reversible disease after tooth implantation, caused by a variety of pathogenic microorganisms. Based on non-surgical or surgical treatment principles, supplementation by local or systemic drugs might enhance treatment efficacy. Porphyromonas gingivalis (Pg) (ATCC 33,277) and Prevotella intermedius (Pi) (ATCC 25,611) were used as test strains. The effects of Pln 149 on the biofilm formation and growth of four periodontal pathogens were evaluated by RT-PCR, fluorescence microscopy, and scanning electron microscopy. The antibacterial mechanism was tested by the patch-clamp technique. The cytotoxicity of Pln 149 (125 µg/ml) to bone marrow stromal cell (BMSC) was assessed using an MTT assay. Pln 149 exhibited significant inhibitory effects on Pg and Pi (P < 0.05), with significant differences in the biofilm images of fluorescence microscope and scanning electron microscope (P < 0.05). Pln 149 could change the sodium channel currents and exerted no cytotoxicity on bone marrow stromal cell. Pln 149 could inhibit the biofilm formation and growth of periodontal pathogens. Considering the absence of antimicrobial resistance and cytotoxicity, we suggest that the Pln 149 from Lactobacillus plantarum 149 might be a promising option for managing peri-implantitis.