Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma
Metastasis, a major challenge during the treatment of lung cancer, causes deterioration in patient health outcomes. Thus, to address this problem, this study aimed to explore the role and contribution of Cholesterol 25-Hydroxylase ( CH25H ) as a potential diagnostic and prognostic marker in lung can...
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Published in | Scientific reports Vol. 12; no. 1; p. 22201 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
23.12.2022
Nature Publishing Group Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | Metastasis, a major challenge during the treatment of lung cancer, causes deterioration in patient health outcomes. Thus, to address this problem, this study aimed to explore the role and contribution of Cholesterol 25-Hydroxylase (
CH25H
) as a potential diagnostic and prognostic marker in lung cancer. Online public databases were used to analyze the expression level, prognostic value, gene-pathway enrichment, and immune infiltration of
CH25H
in lung cancer patients. The Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) was used to analyze and detect the
CH25H
expression levels in leukocytes from lung cancer patients. The expression level of
CH25H
was significantly reduced in lung adenocarcinoma (LUAD), which is associated with a higher disease stage, but not in lung squamous cell carcinoma (LUSC). Kaplan–Meier survival analysis indicated that LUAD patients with low
CH25H
expression had a worse prognosis. Mechanistically, our results showed that in LUAD,
CH25H
may be a regulatory factor affecting the immune cell infiltration level, and the resultant tumor development. Experimental data showed that low expression of
CH25H
in leukocytes was significantly associated with LUAD metastasis (P < 0.01). Our study suggests that
CH25H
may function as a prognostic and risk stratification biomarker for LUAD. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-022-24183-9 |