Microbially facilitated nitrogen cycling in tropical corals

• Published in: The ISME Journal Vol. 16; no. 1; pp. 68 - 77
• Main Authors: Glaze, Thomas D., Erler, Dirk V., Siljanen, Henri. M. P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2022; Oxford University Press
• Subjects: 42/40; 45; 45/22; 45/41; 631/326/47; 64; 704/158/2466; 704/158/47; 704/158/855; 704/47; Ammonium; Ammonium Compounds; Animals; Anthozoa; Biomedical and Life Sciences; Coral reefs; Corals; Cycles; Denitrification; Ecology; Evolutionary Biology; Life Sciences; Mathematical analysis; Metagenomics; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiomes; Microorganisms; Nitrate reduction; Nitrates; Nitrification; Nitrogen; Nitrogen cycle; Nitrogen fixation; Nitrogenation; Nutrient uptake; Regeneration; Stable isotopes; Symbionts
• ISSN: 1751-7362; 1751-7370; 1751-7370
• DOI: 10.1038/s41396-021-01038-1

Tropical scleractinian corals support a diverse assemblage of microbial symbionts. This ‘microbiome’ possesses the requisite functional diversity to conduct a range of nitrogen (N) transformations including denitrification, nitrification, nitrogen fixation and dissimilatory nitrate reduction to ammonium (DNRA). Very little direct evidence has been presented to date verifying that these processes are active within tropical corals. Here we use a combination of stable isotope techniques, nutrient uptake calculations and captured metagenomics to quantify rates of nitrogen cycling processes in a selection of tropical scleractinian corals. Denitrification activity was detected in all species, albeit with very low rates, signifying limited importance in holobiont N removal. Relatively greater nitrogen fixation activity confirms that corals are net N importers to reef systems. Low net nitrification activity suggests limited N regeneration capacity; however substantial gross nitrification activity may be concealed through nitrate consumption. Based on nrfA gene abundance and measured inorganic N fluxes, we calculated significant DNRA activity in the studied corals, which has important implications for coral reef N cycling and warrants more targeted investigation. Through the quantification and characterisation of all relevant N-cycling processes, this study provides clarity on the subject of tropical coral-associated biogeochemical N-cycling.