Tissue fluidity mediated by adherens junction dynamics promotes planar cell polarity-driven ommatidial rotation

• Published in: Nature communications Vol. 12; no. 1; p. 6974
• Main Authors: Founounou, Nabila, Farhadifar, Reza, Collu, Giovanna M., Weber, Ursula, Shelley, Michael J., Mlodzik, Marek
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.11.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/95; 14; 14/1; 14/19; 14/63; 631/136/1660/1993; 631/136/756/1446; 631/80/79/2028; Adherens Junctions - genetics; Adherens Junctions - metabolism; Animals; Cadherins; Cell Polarity - genetics; Cell Polarity - physiology; Drosophila; Drosophila - genetics; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; E-cadherin; Ectoderm; Extracellular Fluid; Eye - cytology; Fluidity; Fruit flies; Humanities and Social Sciences; Insects; Kinases; Mechanical properties; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Morphogenesis; Motility; multidisciplinary; Ommatidia; Polarity; Rotating fluids; Rotation; Science; Science (multidisciplinary); Substrates; Tissues; Vertebrates; Viscoelasticity; Viscosity; Wings, Animal - cytology
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-27253-0

The phenomenon of tissue fluidity—cells’ ability to rearrange relative to each other in confluent tissues—has been linked to several morphogenetic processes and diseases, yet few molecular regulators of tissue fluidity are known. Ommatidial rotation (OR), directed by planar cell polarity signaling, occurs during Drosophila eye morphogenesis and shares many features with polarized cellular migration in vertebrates. We utilize in vivo live imaging analysis tools to quantify dynamic cellular morphologies during OR, revealing that OR is driven autonomously by ommatidial cell clusters rotating in successive pulses within a permissive substrate. Through analysis of a rotation-specific nemo mutant, we demonstrate that precise regulation of junctional E-cadherin levels is critical for modulating the mechanical properties of the tissue to allow rotation to progress. Our study defines Nemo as a molecular tool to induce a transition from solid-like tissues to more viscoelastic tissues broadening our molecular understanding of tissue fluidity. Ommatidial rotation in the Drosophila eye is a regulated process and a Planar Cell Polarity (PCP) cell motility model. Here, the authors show that tissue fluidity via junctional remodeling, as regulated by the PCP effector kinase Nemo, is critical for this cell motility process.