A rotationally focused flow (RFF) microfluidic biosensor by density difference for early-stage detectable diagnosis

• Published in: Scientific reports Vol. 11; no. 1; p. 9277
• Main Authors: Kim, Noori, Han, Kyungsup, Su, Pei-Chen, Kim, Insup, Yoon, Yong-Jin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.04.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 639/166/988; 639/301/1005/1009; Biomarkers; Biomarkers - metabolism; Biosensing Techniques; Biosensors; Biotin; Coronaviruses; COVID-19; COVID-19 - diagnosis; Diagnosis; Early Diagnosis; Humanities and Social Sciences; Humans; Lab-on-a-chip; Lab-On-A-Chip Devices; Microfluidics; Microfluidics - methods; multidisciplinary; Pandemics; SARS-CoV-2 - physiology; Science; Science (multidisciplinary); Sensitivity and Specificity; Streptavidin
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-88647-0

Label-free optical biosensors have received tremendous attention in point-of-care testing, especially in the emerging pandemic, COVID-19, since they advance toward early-detection, rapid, real-time, ease-of-use, and low-cost paradigms. Protein biomarkers testings require less sample modification process compared to nucleic-acid biomarkers’. However, challenges always are in detecting low-concentration for early-stage diagnosis. Here we present a Rotationally Focused Flow (RFF) method to enhance sensitivity(wavelength shift) of label-free optical sensors by increasing the detection probability of protein-based molecules. The RFF is structured by adding a less-dense fluid to focus the target-fluid in a T-shaped microchannel. It is integrated with label-free silicon microring resonators interacting with biotin-streptavidin. The suggested mechanism has demonstrated 0.19 fM concentration detection along with a significant magnitudes sensitivity enhancement compared to single flow methods. Verified by both CFD simulations and fluorescent flow-experiments, this study provides a promising proof-of-concept platform for next-generation lab-on-a-chip bioanalytics such as ultrafast and early-detection of COVID-19.