A large accessory protein interactome is rewired across environments
To characterize how protein-protein interaction (PPI) networks change, we quantified the relative PPI abundance of 1.6 million protein pairs in the yeast across nine growth conditions, with replication, for a total of 44 million measurements. Our multi-condition screen identified 13,764 pairwise PPI...
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Published in | eLife Vol. 9 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
eLife Sciences Publications, Ltd
14.09.2020
eLife Sciences Publications Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | To characterize how protein-protein interaction (PPI) networks change, we quantified the relative PPI abundance of 1.6 million protein pairs in the yeast
across nine growth conditions, with replication, for a total of 44 million measurements. Our multi-condition screen identified 13,764 pairwise PPIs, a threefold increase over PPIs identified in one condition. A few 'immutable' PPIs are present across all conditions, while most 'mutable' PPIs are rarely observed. Immutable PPIs aggregate into highly connected 'core' network modules, with most network remodeling occurring within a loosely connected 'accessory' module. Mutable PPIs are less likely to co-express, co-localize, and be explained by simple mass action kinetics, and more likely to contain proteins with intrinsically disordered regions, implying that environment-dependent association and binding is critical to cellular adaptation. Our results show that protein interactomes are larger than previously thought and contain highly dynamic regions that reorganize to drive or respond to cellular changes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE Office of Science (SC) Louis and Beatrice Laufer Center New York State Center for Biotechnology National Institutes of Health (NIH) Joint Initiative for Metrology in Biology AC02-76SF00515; R01HG008354 |
ISSN: | 2050-084X 2050-084X |
DOI: | 10.7554/elife.62365 |