Effect of α-tocopherol on lipid peroxidation and total antioxidant status in spontaneously hypertensive rats

• Published in: American journal of hypertension Vol. 11; no. 12; pp. 1480 - 1485
• Main Authors: Newaz, M.A, Nawal, N.N.A
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.1998; Oxford University Press; Elsevier Science
• Subjects: Animals; antioxidant status; Antioxidants - pharmacology; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; blood pressure; Cardiology. Vascular system; Clinical manifestations. Epidemiology. Investigative techniques. Etiology; Free radical; Hypertension - drug therapy; Hypertension - metabolism; Lipid Peroxidation - drug effects; lipid peroxide; Male; Medical sciences; Rats; Rats, Inbred SHR; Rats, Inbred WKY; superoxide dismutase; Superoxide Dismutase - metabolism; Tropical medicine; Vitamin E - pharmacology; Vitamin E - therapeutic use; blood pressure; antioxidant status; superoxide dismutase; Free radical; lipid peroxide; Hypertension; Rat; Enzyme; Rodentia; Cardiovascular disease; Exploration; Lipids; Superoxide dismutase; α-Tocopherol; Antioxidant; Genetic disease; Vertebrata; Mammalia; Enzymatic activity; Animal; Blood pressure; Oxidoreductases; Supplementation; Comparative study; Peroxidation
• ISSN: 0895-7061; 1879-1905; 1941-7225
• DOI: 10.1016/S0895-7061(98)00167-8

The aim of this study was to determine the effects of α-tocopherol on lipid peroxidation and total antioxidant status of spontaneously hypertensive rats (SHR), comparing them with normal Wistar-Kyoto (WKY) rats. SHR were divided into three groups and treated with different doses of α-tocopherol (α 1, 17 mg/kg diet; α 2, 34 mg/kg diet; and α 3, 170 mg/kg diet). Normal WKY and untreated SHR were used as normal (N) and hypertensive control (HC). Blood pressures were recorded every 10 days for 3 months. At the end of the trial, animals were killed and measurement of plasma total antioxidant status, plasma superoxide dismutase (SOD) activity, and lipid peroxide levels in plasma and blood vessels was carried out following well-established methods. From our study it was found that lipid peroxides in thoracic aorta (N, 0.47 ± 0.17; H, 0.96 ± 0.37; P < .0001) and plasma (N, 0.06 ± 0.01; H, 0.13 ± 0.01) were significantly higher in hypertensives than in normal rats. SOD activity was significantly lower in hypertensive than normal rats (N, 172.93 ± 46.91; H, 110.08 ± 14.38; P < .005). Total antioxidant status was significantly higher in normal than hypertensive rats (N, 0.88 ± 0.05; H, 0.83 ± 0.02; P < .05). After the antioxidant trial, it was found that in the treated groups rise of blood pressure was prevented significantly ( P < .001) and lipid peroxides in blood vessels were significantly reduced more than in the controls ( P < .001). For plasma lipid peroxide it was only significant for groups α 2 ( P < .001) and α 3 ( P < .05). Although all three treated groups showed improved total antioxidant status, only groups α 2 (0.87 ± 0.04, P < .005) and α 3 (1.20 ± 0.18, P < .001) were statistically significant. All the three groups showed significant increases in their SOD activity ( P < .001). Correlation studies showed that total antioxidant status and SOD were significantly negatively correlated with blood pressure in normal rats ( P = .007; P = .008). Lipid peroxides in both blood vessel and plasma showed a positive correlation. In the treated groups, lipid peroxides in blood vessels maintained a significant positive correlation with blood pressure in all groups (α 1, P = .021; α 2, P = .019; α 3, P = .002), whereas for plasma lipid peroxides the correlation was in groups α 1 ( P = .005) and α 2 ( P = .009). For SOD activity, significant negative correlations were found with blood pressure in the α 2 ( P = .017) and α 3 ( P = .025) groups. Total antioxidant status maintained a significant negative correlation with blood pressure in all three groups (α 1, P = .012; α 2, P = .044; α 3, P = .014). In conclusion it was found that supplement of α-tocopherol may prevent development of increased blood pressure, reduce lipid peroxides in plasma and blood vessels, and enhance the total antioxidant status, including SOD activity.