Imaging biological tissue with high-throughput single-pixel compressive holography

• Published in: Nature communications Vol. 12; no. 1; pp. 4712 - 12
• Main Authors: Wu, Daixuan, Luo, Jiawei, Huang, Guoqiang, Feng, Yuanhua, Feng, Xiaohua, Zhang, Runsen, Shen, Yuecheng, Li, Zhaohui
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.08.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/245; 639/624/1107/328/1650; 639/624/1107/510; Animals; Arrays; Brain - anatomy & histology; Data Compression - methods; Data Compression - statistics & numerical data; Detectors; Female; Field of view; High resolution; Holography; Holography - instrumentation; Holography - methods; Holography - statistics & numerical data; Humanities and Social Sciences; Image contrast; Image Processing, Computer-Assisted - methods; Image Processing, Computer-Assisted - statistics & numerical data; Image resolution; Mice; Mice, Inbred C57BL; Mice, Nude; multidisciplinary; Noise sensitivity; Optical Devices; Optical Imaging - instrumentation; Optical Imaging - methods; Optical Imaging - statistics & numerical data; Optical Phenomena; Phase stepping; Pixels; Science; Science (multidisciplinary); Sensors; Spatial data; Tail - anatomy & histology; Tissues; Visual field; Wavelengths
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-24990-0

Single-pixel holography (SPH) is capable of generating holographic images with rich spatial information by employing only a single-pixel detector. Thanks to the relatively low dark-noise production, high sensitivity, large bandwidth, and cheap price of single-pixel detectors in comparison to pixel-array detectors, SPH is becoming an attractive imaging modality at wavelengths where pixel-array detectors are not available or prohibitively expensive. In this work, we develop a high-throughput single-pixel compressive holography with a space-bandwidth- time product (SBP-T) of 41,667 pixels/s, realized by enabling phase stepping naturally in time and abandoning the need for phase-encoded illumination. This holographic system is scalable to provide either a large field of view (~83 mm 2 ) or a high resolution (5.80 μm × 4.31 μm). In particular, high-resolution holographic images of biological tissues are presented, exhibiting rich contrast in both amplitude and phase. This work is an important step towards multi-spectrum imaging using a single-pixel detector in biophotonics. Single-pixel holography generates holographic images with a single-pixel detector making this relatively inexpensive. Here the authors report a high-throughput single-pixel compressive holography method for imaging biological tissue which can either provide a large field of view or high resolution.