OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
Background During anther development, the tapetum provides essential nutrients and materials for pollen development. In rice, multiple transcription factors and enzymes essential for tapetum development and pollen wall formation have been cloned from male-sterile lines. Results In this study, we obt...
Saved in:
Published in | Rice (New York, N.Y.) Vol. 14; no. 1; p. 4 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
06.01.2021
Springer Nature B.V SpringerOpen |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Background
During anther development, the tapetum provides essential nutrients and materials for pollen development. In rice, multiple transcription factors and enzymes essential for tapetum development and pollen wall formation have been cloned from male-sterile lines.
Results
In this study, we obtained several lines in which the MYB transcription factor
OsMS188
was knocked out through the CRISPR-Cas9 approach. The
osms188
lines exhibited a male-sterile phenotype with aberrant development and degeneration of tapetal cells, absence of the sexine layer and defective anther cuticles.
CYP703A3
,
CYP704B2
,
OsPKS1
,
OsPKS2
,
DPW
and
ABCG15
are sporopollenin synthesis and transport-related genes in rice. Plants with mutations in these genes are male sterile, with a defective sexine layer and anther cuticle. Further biochemical assays demonstrated that OsMS188 binds directly to the promoters of these genes to regulate their expression.
UDT1
,
OsTDF1
,
TDR
,
bHLH142
and
EAT1
are upstream regulators of rice tapetum development. Electrophoretic mobility shift assays (EMSAs) and activation assays revealed that TDR directly regulates
OsMS188
expression. Additionally, protein interaction assays indicated that TDR interacts with OsMS188 to regulate downstream gene expression.
Conclusion
Overall, OsMS188 is a key regulator of tapetum development and pollen wall formation. The gene regulatory network established in this work may facilitate future investigations of fertility regulation in rice and in other crop species. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1939-8425 1939-8433 1934-8037 |
DOI: | 10.1186/s12284-020-00451-y |