APPLICATIONS OF CAPILLARY ELECTROPHORESIS TO REDOX PROTEINS AND NUCLEOTIDES IN PHOTOSYNTHETIC SYSTEM

Several redox proteins and nucleotides involved in photosynthetic electron transfer were separated by capillary zone electrophoresis (CZE) with a noncoated fused silica capillary column. We found that reduced and oxidized forms of plastocyanin, cytochrome c, NAD and NADP could be separated by CZE. T...

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Published inAnalytical Sciences Vol. 7; no. Supple; pp. 1545 - 1548
Main Authors SENDA, MASAAKI, SASAKI, TORU, NAKAMOTO, HITOSHI, HIYAMA, TETSUO
Format Journal Article
LanguageEnglish
Published The Japan Society for Analytical Chemistry 1991
Subjects
Capillary electrophoresis
membrane proteins
nicotinamide nucleotides
photosynthesis
redox proteins
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Summary:Several redox proteins and nucleotides involved in photosynthetic electron transfer were separated by capillary zone electrophoresis (CZE) with a noncoated fused silica capillary column. We found that reduced and oxidized forms of plastocyanin, cytochrome c, NAD and NADP could be separated by CZE. Two types of ferredoxin, commonly present among higher plants, were also clearly separated by this technique.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.7.Supple_1545