Dynamics, phylogeny and phyto-stimulating potential of chitinase synthesizing bacterial root endosymbiosiome of North Western Himalayan Brassica rapa L

• Published in: Scientific reports Vol. 12; no. 1; p. 6742
• Main Authors: Padder, Shahid Ahmad, Rather, Rauoof Ahmad, Bhat, Sajad Ahmad, Shah, M. D., Baba, Tawseef Rehman, Mubarak, N. M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.04.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647; 631/326; 631/449; 631/61; Acetic acid; Amikacin; Ammonia; Bacteria; Biological control; Brassica rapa; Calcium phosphates; Carbon sources; Chitinase; Chitinases; Chloramphenicol; Diversity indices; Endophytes; Erythromycin; Farming systems; Germplasm; Glycerol; Humanities and Social Sciences; Indoleacetic acid; Microflora; multidisciplinary; Pesticides; Phylogeny; Plant growth; Plant Roots - microbiology; Rhizosphere; Rifampin; RNA, Ribosomal, 16S; Science; Science (multidisciplinary); Sequence analysis; Siderophores; Species diversity; Strains (organisms); Sustainable agriculture; Tricalcium phosphate
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-11030-0

The less phytopathogen susceptibility in Himalayan Brassica rapa L. has made it an exceptional crop eluding synthetic pesticide inputs, thereby guarantying economically well-founded and ecologically sustainable agriculture. The relevance of niche microflora of this crop has not been deliberated in this context, as endosymbiosiome is more stable than their rhizosphere counterparts on account of their restricted acquaintance with altering environment; therefore, the present investigation was carried out to study the endophytic microfloral dynamics across the B . rapa germplasm in context to their ability to produce chitinase and to characterize the screened microflora for functional and biochemical comportments in relevance to plant growth stimulation. A total of 200 colonies of bacterial endophytes were isolated from the roots of B . rapa across the J&K UT, comprising 66 locations. After morphological, ARDRA, and sequence analysis, eighty-one isolates were selected for the study, among the isolated microflora Pseudomonas sp. Bacillus sp. dominated. Likewise, class γ-proteobacteria dominated, followed by Firmicutes. The diversity studies have exposed changing fallouts on all the critical diversity indices, and while screening the isolated microflora for chitinase production, twenty-two strains pertaining to different genera produced chitinase. After carbon source supplementation to the chitinase production media, the average chitinase activity was significantly highest in glycerol supplementation. These 22 strains were further studied, and upon screening them for their fungistatic behavior against six fungal species, wide diversity was observed in this context. The antibiotic sensitivity pattern of the isolated strains against chloramphenicol, rifampicin, amikacin, erythromycin, and polymyxin-B showed that the strains were primarily sensitive to chloramphenicol and erythromycin. Among all the strains, only eleven produced indole acetic acid, ten were able to solubilize tricalcium phosphate and eight produced siderophores. The hydrocyanic acid and ammonia production was observed in seven strains each. Thus, the present investigation revealed that these strains could be used as potential plant growth promoters in sustainable agriculture systems besides putative biocontrol agents.