QTL associated with Gummy Stem Blight (GSB) resistance in watermelon

• Published in: BMC genomics Vol. 23; no. 1; pp. 1 - 632
• Main Authors: Hong, Jeong-Eui, Hossain, Mohammad Rashed, Jung, Hee-Jeong, Nou, Ill-Sup
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 03.09.2022; BioMed Central; BMC
• Subjects: Analysis; Blight; Chromosome 1; Citrullus lanatus; Cultivars; Didymella bryoniae; Disease; Disease resistance; Diseases and pests; Fruits; Gene mapping; Genes; Genetic aspects; Genomes; Genomics; Genotype & phenotype; Genotyping; Gummy stem blight; Heredity; Humidity; Leaves; Nucleotides; Pathogens; Phenotypic variations; Plant immunology; QTL; Quantitative trait loci; Signs and symptoms; Single-nucleotide polymorphism; Stagonosporopsis cucurbitacearum; Stem blight; Transcription factors; Water melons; Watermelon; Watermelons; South Korea; United States > US; Germany
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-022-08849-2

Abstract Background Gummy stem blight (GSB), caused by Didymella bryoniae (syn. Stagonosporopsis cucurbitacearum ), produces devastating symptoms on whole plants of watermelon ( Citrullus lanatus ) and other cucurbits, significantly reducing yield and quality. Identification of genetic determinants and sources of resistance to this devastating GSB disease in watermelon is essential for developing resistant varieties. Results In this study, we aimed at identifying quantitative trait loci (QTLs) linked to GSB resistance in melon. We identified the genome-wide single nucleotide polymorphisms (SNPs) by genotyping by sequencing (GBS) of an F 2 population developed from C. lanatus lines, ‘PI 279461’ ( resistant ) ✕ ‘PI 223764’ (susceptible). Inheritance analysis indicated that resistance to GSB is a multi-genic trait in this population. Three QTLs namely, ClGSB1.1 , ClGSB10.1 , and ClGSB11.1 associated with GSB resistance, explaining approximately 10% of the phenotypic variation, were identified. Among these, the QTL ClGSB1.1 on chromosome 1 is identified as a major QTL harboring five candidate genes associated with GSB resistance including two RLKs ( ClC01G014900 and ClC01G015010 ), two WRKY transcription factors ( ClC01G014910 and ClC01G014990 ), and one AvrRpt-cleavage domain protein ( ClC01G015130 ). Conclusion Two high resolution melting (HRM) markers, WmGSB1.1–2 and WmGSB1.1–7 having a high positive correlation with the phenotypic variations, were developed. Five potential candidate genes were predicted to be associated with GSB resistance. These findings will help breeders to develop watermelon cultivars resistant to GSB.