Exploring the Modulatory Effect of High-Fat Nutrition on Lipopolysaccharide-Induced Acute Lung Injury in Vagotomized Rats and the Role of the Vagus Nerve

• Published in: Nutrients Vol. 15; no. 10; p. 2327
• Main Authors: Seesing, Maarten F J, Janssen, Henricus J B, Geraedts, Tessa C M, Weijs, Teus J, van Ark, Ingrid, Leusink-Muis, Thea, Folkerts, Gert, Garssen, Johan, Ruurda, Jelle P, Nieuwenhuijzen, Grard A P, van Hillegersberg, Richard, Luyer, Misha D P
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.05.2023; MDPI
• Subjects: Abdomen; acetylcholine; Acetylcholine receptors (nicotinic); Acute Lung Injury - metabolism; Acute respiratory distress syndrome; agonist; Agonists; alpha7 Nicotinic Acetylcholine Receptor - metabolism; Animals; Aprotinin; Bronchus; Carbohydrates; cholecystokinin; Complications; Complications and side effects; Enteral nutrition; Ethylenediaminetetraacetic acid; Experiments; Fasting; high-fat; Inflammation; Injury prevention; Interleukin 6; Laboratory animals; Lavage; Lipopolysaccharides; Lipopolysaccharides - metabolism; Lungs; Macrophages; nerve stimulation; Nutrition; Ostomy; Postoperative; Pulmonary functions; Rats; Respiratory function; Tumor necrosis factor-α; Vagotomy; Vagus nerve; Vagus Nerve - metabolism; France; Netherlands; Paris France; vagus nerve; nerve stimulation; high-fat; inflammation; vagotomy; cholecystokinin; agonist; acetylcholine; rats
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu15102327

During esophagectomy, the vagus nerve is transected, which may add to the development of postoperative complications. The vagus nerve has been shown to attenuate inflammation and can be activated by a high-fat nutrition via the release of acetylcholine. This binds to α7 nicotinic acetylcholine receptors (α7nAChR) and inhibits α7nAChR-expressing inflammatory cells. This study investigates the role of the vagus nerve and the effect of high-fat nutrition on lipopolysaccharide (LPS)-induced lung injury in rats. Firstly, 48 rats were randomized in 4 groups as follows: sham (sparing vagus nerve), abdominal (selective) vagotomy, cervical vagotomy and cervical vagotomy with an α7nAChR-agonist. Secondly, 24 rats were randomized in 3 groups as follows: sham, sham with an α7nAChR-antagonist and cervical vagotomy with an α7nAChR-antagonist. Finally, 24 rats were randomized in 3 groups as follows: fasting, high-fat nutrition before sham and high-fat nutrition before selective vagotomy. Abdominal (selective) vagotomy did not impact histopathological lung injury (LIS) compared with the control (sham) group ( > 0.999). There was a trend in aggravation of LIS after cervical vagotomy ( = 0.051), even after an α7nAChR-agonist ( = 0.090). Cervical vagotomy with an α7nAChR-antagonist aggravated lung injury ( = 0.004). Furthermore, cervical vagotomy increased macrophages in bronchoalveolar lavage (BAL) fluid and negatively impacted pulmonary function. Other inflammatory cells, TNF-α and IL-6, in the BALF and serum were unaffected. High-fat nutrition reduced LIS after sham ( = 0.012) and selective vagotomy ( = 0.002) compared to fasting. vagotomy. This study underlines the role of the vagus nerve in lung injury and shows that vagus nerve stimulation using high-fat nutrition is effective in reducing lung injury, even after selective vagotomy.