The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
Histone deacetylation is one of the well characterized post-translational modifications related to transcriptional repression in eukaryotes. The process of histone deacetylation is achieved by histone deacetylases (HDACs). Over the last decade, substantial advances in our understanding of the mechan...
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Published in | Scientific reports Vol. 7; no. 1; pp. 7930 - 10 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
11.08.2017
Nature Publishing Group Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | Histone deacetylation is one of the well characterized post-translational modifications related to transcriptional repression in eukaryotes. The process of histone deacetylation is achieved by histone deacetylases (HDACs). Over the last decade, substantial advances in our understanding of the mechanism of fruit ripening have been achieved, but the role of
HDACs
in this process has not been elucidated. In our study, an RNA interference (RNAi) expression vector targeting
SlHDA1
was constructed and transformed into tomato plants. Shorter fruit ripening time and decreased storability were observed in
SlHDA1
RNAi lines. The accumulation of carotenoid was increased through an alteration of the carotenoid pathway flux. Ethylene content, ethylene biosynthesis genes (
ACS2
,
ACS4
and
ACO1
,
ACO3
) and ripening-associated genes (
RIN
,
E4
,
E8
,
Cnr
,
TAGL1
,
PG
,
Pti4
and
LOXB
) were significantly up-regulated in
SlHDA1
RNAi lines. In addition, the expression of fruit cell wall metabolism genes (
HEX
,
MAN
,
TBG4
,
XTH5
and
XYL
) was enhanced compared with wild type. Furthermore,
SlHDA1
RNAi seedlings displayed shorter hypocotyls and were more sensitive to ACC (1-aminocyclopropane-1-carboxylate) than the wild type. The results of our study indicate that
SlHDA1
functions as a negative regulator of fruit ripening by affecting ethylene synthesis and carotenoid accumulation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-08512-x |