Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice

• Published in: Nature communications Vol. 10; no. 1; pp. 1431 - 15
• Main Authors: Edfawy, Mohamed, Guedes, Joana R., Pereira, Marta I., Laranjo, Mariana, Carvalho, Mário J., Gao, Xian, Ferreira, Pedro A., Caldeira, Gladys, Franco, Lara O., Wang, Dongqing, Cardoso, Ana Luisa, Feng, Guoping, Carvalho, Ana Luisa, Peça, João
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.03.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 13/106; 13/109; 14/19; 14/28; 38/44; 631/378/1689/1373; 631/378/3919; 64/60; Animals; Autism; Autistic Disorder - genetics; Autistic Disorder - psychology; Behavior, Animal; Dendritic spines; Dendritic Spines - pathology; Dendritic Spines - ultrastructure; Disabilities; G protein-coupled receptors; Gene Deletion; Glutamatergic transmission; Glutamic acid receptors (metabotropic); Hippocampus; Hippocampus - pathology; Humanities and Social Sciences; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - physiology; Long-term depression; Maturation; Memory; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Mutant Strains; Morbidity; multidisciplinary; Neurodevelopmental disorders; Neuronal Plasticity - genetics; Plasticity; Protein transport; Proteins; Receptor, Metabotropic Glutamate 5 - metabolism; Receptors; Science; Science (multidisciplinary); Social behavior; Social factors; Social interactions; Spine; Synapses; Synaptic density; Synaptic plasticity; Synaptic Transmission
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-09382-9

Autism spectrum disorder (ASD) is characterized by dysfunction in social interactions, stereotypical behaviours and high co-morbidity with intellectual disability. A variety of syndromic and non-syndromic neurodevelopmental disorders have been connected to alterations in metabotropic glutamate receptor (mGluR) signalling. These receptors contribute to synaptic plasticity, spine maturation and circuit development. Here, we investigate the physiological role of Gprasp2 , a gene linked to neurodevelopmental disabilities and involved in the postendocytic sorting of G-protein-coupled receptors. We show that Gprasp2 deletion leads to ASD-like behaviour in mice and alterations in synaptic communication. Manipulating the levels of Gprasp2 bidirectionally modulates the surface availability of mGluR 5 and produces alterations in dendritic complexity, spine density and synaptic maturation. Loss of Gprasp2 leads to enhanced hippocampal long-term depression, consistent with facilitated mGluR-dependent activation. These findings demonstrate a role for Gprasp2 in glutamatergic synapses and suggest a possible mechanism by which this gene is linked to neurodevelopmental diseases. GPRASP2 plays a role in trafficking of GPCRs and mutations in this gene have been linked to neurodevelopmental disorders. Here the authors study the role of Gprasp2 in the CNS and show that it regulates the surface availability of mGluR5 receptors and that knockout mice for this protein show autistic-like behavioural abnormalities.