Novel Size-Variable Dedicated Rodent Oxygenator for ECLS Animal Models-Introduction of the "RatOx" Oxygenator and Preliminary In Vitro Results

• Published in: Micromachines (Basel) Vol. 14; no. 4; p. 800
• Main Authors: Strudthoff, Lasse J, Focke, Jannis, Hesselmann, Felix, Kaesler, Andreas, Martins Costa, Ana, Schlanstein, Peter C, Schmitz-Rode, Thomas, Steinseifer, Ulrich, Steuer, Niklas B, Wiegmann, Bettina, Arens, Jutta, Jansen, Sebastian V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.03.2023; MDPI
• Subjects: animal model; Blood flow; Blood oxygenation, Extracorporeal; Coronaviruses; COVID-19; Design; Design and construction; ECLS; ECMO; Extracorporeal membrane oxygenation; Medical research; Membranes; Modules; mouse; Oxygenators; Oxygenators, Membrane; Pandemics; Patients; Priming; R&D; rat; Research & development; rodent; Surgical apparatus & instruments; Germany; ECMO; mouse; CPB; rat; ECLS; hamster; oxygenator; rodent; in vivo; animal model
• ISSN: 2072-666X; 2072-666X
• DOI: 10.3390/mi14040800

The overall survival rate of extracorporeal life support (ECLS) remains at 60%. Research and development has been slow, in part due to the lack of sophisticated experimental models. This publication introduces a dedicated rodent oxygenator ("RatOx") and presents preliminary in vitro classification tests. The RatOx has an adaptable fiber module size for various rodent models. Gas transfer performances over the fiber module for different blood flows and fiber module sizes were tested according to DIN EN ISO 7199. At the maximum possible amount of effective fiber surface area and a blood flow of 100 mL/min, the oxygenator performance was tested to a maximum of 6.27 mL O /min and 8.2 mL CO /min, respectively. The priming volume for the largest fiber module is 5.4 mL, while the smallest possible configuration with a single fiber mat layer has a priming volume of 1.1 mL. The novel RatOx ECLS system has been evaluated in vitro and has demonstrated a high degree of compliance with all pre-defined functional criteria for rodent-sized animal models. We intend for the RatOx to become a standard testing platform for scientific studies on ECLS therapy and technology.