An insight into synthesis and antitumor activity of citrate and gallate stabilizing gold nanospheres

• Published in: Scientific reports Vol. 13; no. 1; p. 2749
• Main Authors: Fathy, Mohamed M., Elfiky, Abdo A., Bashandy, Yousef S., Hamdy, Mayar M., Elgharib, Ahmed M., Ibrahim, Ibrahim M., Kamal, Rana T., Mohamed, Ahmed S., Rashad, Anan M., Ahmed, Ola S., Elkaramany, Yomna, Abdelaziz, Youssef S., Amin, Fatma G., Eid, Jehane I.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.02.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/337; 631/57; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Antitumor agents; Bioassays; Biocompatibility; Cancer; Cell cycle; Cervical cancer; Chemical reduction; Chemotherapy; Citrates; Citric acid; Citric Acid - chemistry; Comet assay; Cytotoxicity; Drug development; Flow cytometry; Gallic acid; Genotoxicity; Gold; Gold - chemistry; Gold - pharmacology; HeLa Cells; Humanities and Social Sciences; Humans; Metal Nanoparticles - chemistry; Metastases; multidisciplinary; Nanoparticles; Nanospheres; Physical properties; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-29821-4

Both gallic and citrate are well-established antioxidants that show promise as new selective anti-cancer drugs. Gold nanoparticles (AuNPs) as well can be developed as flexible and nontoxic nano-carriers for anti-cancer drugs. This article evaluating the efficiency and biocompatibility of gallic acid and citrate capping gold nanoparticles to be used as anti-cancer drug. The biosafety and therapeutic efficiency of prepared nano-formulations were tested on Hela and normal BHK cell line. Gold nanospheres coated with citrate and gallate were synthesized via wet chemical reduction method. The prepared nano-formulations, citrate and gallate coated gold nanospheres (Cit-AuNPs and Ga-AuNPs), were characterized with respect to their morphology, FTIR spectra, and physical properties. In addition, to assess their cytotoxicity, cell cycle arrest and flow cytometry to measure biological response were performed. Cit-Au NPs and Ga-Au NPs were shown to significantly reduce the viability of Hela cancer cells. Both G0/G cell cycle arrest and comet assay results showed that genotoxic effect was induced in Hela cells by Cit-Au NPs and Ga-Au NPs. The results of this study showed that Cit-Au NPs and Ga-AuNPs inhibit the growth of metastatic cervical cancer cells, which could have therapeutic implications.