Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells

• Published in: Experimental & molecular medicine Vol. 42; no. 12; pp. 805 - 810
• Main Authors: Kim, Woon-Ki, Sul, Ok-Ju, Kwak, Jung-Sook, Hur, Hye-Young, Latour, Anne M, Koller, Beverly H, Kwon, Byoung S, Jeong, Choon-Soo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31.12.2010; Springer Nature B.V; Korean Society for Biochemistry and Molecular Biology; 생화학분자생물학회
• Subjects: Animals; Base Sequence; Biomedical and Life Sciences; Biomedicine; CD4-Positive T-Lymphocytes - metabolism; Cells, Cultured; Down-Regulation; Medical Biochemistry; Mice; Mice, Inbred C57BL; Molecular Medicine; Molecular Sequence Data; NFATC Transcription Factors - physiology; Original; Receptors, Tumor Necrosis Factor, Member 14 - biosynthesis; Stem Cells; T-Lymphocytes - metabolism; 생화학; gene expression regulation; T lymphocytes; tumor necrosis factor ligand superfamily member 14; NFATC transcription factors; promoter regions, genetic
• ISSN: 1226-3613; 2092-6413
• DOI: 10.3858/emm.2010.42.12.083

Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.