The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein sustain persistent and lytic infections

• Published in: Nature communications Vol. 11; no. 1; p. 1997
• Main Authors: Prasad, Vibhu, Suomalainen, Maarit, Jasiqi, Yllza, Hemmi, Silvio, Hearing, Patrick, Hosie, Louise, Burgert, Hans-Gerhard, Greber, Urs F.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.04.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 14/10; 14/19; 14/35; 38/89; 42/44; 631/326/596/1278; 631/80/304; 96/1; 96/106; 96/95; A549 Cells; Activating transcription factor 6; Adenoviridae - genetics; Adenoviridae - immunology; Adenoviridae - pathogenicity; Adenoviridae Infections - genetics; Adenoviridae Infections - immunology; Adenoviridae Infections - virology; Adenovirus E1A Proteins - genetics; Adenovirus E3 Proteins - metabolism; Adenoviruses; Biological analysis; Chemical interference; Chronic Disease; Chronic illnesses; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Endoribonucleases - genetics; Endoribonucleases - metabolism; Gene Expression Regulation, Viral - immunology; Gene Knockdown Techniques; Gene Knockout Techniques; Genetics; Glycoproteins; HeLa Cells; Host-Pathogen Interactions - genetics; Humanities and Social Sciences; Humans; Immunocompromised Host; Infections; Interferon; Interferon-gamma - genetics; Interferon-gamma - immunology; Interferon-gamma - metabolism; Kinases; Membrane proteins; multidisciplinary; Nuclease; Persistent infection; Protein folding; Protein kinase R; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Proteins; RNA Splicing; Science; Science (multidisciplinary); Sensors; Splicing; Virus Latency; Virus Release - genetics; Viruses; X-Box Binding Protein 1 - genetics
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15844-2

Persistent viruses cause chronic disease, and threaten the lives of immunosuppressed individuals. Here, we elucidate a mechanism supporting the persistence of human adenovirus (AdV), a virus that can kill immunosuppressed patients. Cell biological analyses, genetics and chemical interference demonstrate that one of five AdV membrane proteins, the E3-19K glycoprotein specifically triggers the unfolded protein response (UPR) sensor IRE1α in the endoplasmic reticulum (ER), but not other UPR sensors, such as protein kinase R-like ER kinase (PERK) and activating transcription factor 6 (ATF6). The E3-19K lumenal domain activates the IRE1α nuclease, which initiates mRNA splicing of X-box binding protein-1 (XBP1). XBP1s binds to the viral E1A-enhancer/promoter sequence, and boosts E1A transcription, E3-19K levels and lytic infection. Inhibition of IRE1α nuclease interrupts the five components feedforward loop, E1A, E3-19K, IRE1α, XBP1s, E1A enhancer/promoter. This loop sustains persistent infection in the presence of the immune activator interferon, and lytic infection in the absence of interferon. Adenovirus (AdV) can cause persistent infections, but underlying mechanisms are poorly understood. Here, Prasad et al. show that the AdV glycoprotein E3-19K activates the unfolded protein response sensor IRE1α, and that this triggers a feedforward loop that sustains persistent infection in the presence of interferon.