A temperature sensitive Mycobacterium paragordonae induces enhanced protective immune responses against mycobacterial infections in the mouse model

• Published in: Scientific reports Vol. 7; no. 1; pp. 15230 - 14
• Main Authors: Kim, Byoung-Jun, Kim, Bo-Ram, Kook, Yoon-Hoh, Kim, Bum-Joon
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.11.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/21; 13/31; 631/250; 631/326/590; 64/60; Animals; Antibodies, Bacterial - blood; Bacillus Calmette-Guerin vaccine; BCG; Cytokines - metabolism; Cytotoxicity; Dendritic Cells - immunology; Hot Temperature; Humanities and Social Sciences; Immune response; Intravenous administration; Lymphocytes T; Mice, Inbred BALB C; multidisciplinary; Mutation; Mycobacterium; Mycobacterium Infections, Nontuberculous - prevention & control; Nontuberculous Mycobacteria - genetics; Nontuberculous Mycobacteria - growth & development; Nontuberculous Mycobacteria - immunology; Nontuberculous Mycobacteria - radiation effects; Science; Science (multidisciplinary); T cell receptors; T-Lymphocytes, Cytotoxic - immunology; Temperature effects; Th1 Cells - immunology; Tuberculosis; Tuberculosis - prevention & control; Tuberculosis Vaccines - administration & dosage; Tuberculosis Vaccines - immunology; Tuberculosis Vaccines - isolation & purification; Vaccination; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - immunology; Vaccines, Attenuated - isolation & purification
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-15458-7

Recently, we introduced a temperature sensitive Mycobacterium spp ., Mycobacterium paragordonae (Mpg). Here, we checked its potential as a candidate for live vaccination against Mycobacterium tuberculosis and Mycobacterium abscessus . Intravenous infections of mice with Mpg led to lower colony forming units (CFUs) compared to infection with BCG, suggesting its usefulness as a live vaccine. The analyses of immune responses indicated that the highly protective immunity elicited by Mpg was dependent on effective dendritic maturation, shift of cytokine patterns and antibody production toward a Th1 phenotype, and enhanced cytotoxic T cell response. Compared to BCG, Mpg showed a more effective protective immune response in the vaccinated mice against challenges with 2 different mycobacterial strains, M. tuberculosis H37Ra or M. abscessus Asan 50594. Our data suggest that a temperature sensitive Mpg may be a potentially powerful candidate vaccine strain to induce enhanced protective immune responses against M. tuberculosis and M. abscessus .