Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions

Physiologically, lipopolysaccharide (LPS) is present in the bloodstream and can be bound to several proteins for its transport (i.e.) LPS binding protein (LBP) and plasma lipoproteins). LPS receptors CD14 and TLR-4 are constitutively expressed in the Central Nervous System (CNS). To our knowledge, L...

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Published inScientific reports Vol. 7; no. 1; pp. 13113 - 15
Main Authors Vargas-Caraveo, Alejandra, Sayd, Aline, Maus, Sandra R., Caso, Javier R., Madrigal, José L. M., García-Bueno, Borja, Leza, Juan C.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 13.10.2017
Nature Publishing Group
Subjects
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631/250/256
631/378/371
82/1
82/29
96/63
Apolipoprotein E
Astrocytes
CD14 antigen
Central nervous system
Choroid plexus
Endothelial cells
Humanities and Social Sciences
Interfaces
Lipid A
Lipopolysaccharides
Lipoprotein receptors
Lipoproteins
Localization
Low density lipoprotein receptors
multidisciplinary
Physiology
Rodents
Science
Science (multidisciplinary)
Tanycytes
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Summary:Physiologically, lipopolysaccharide (LPS) is present in the bloodstream and can be bound to several proteins for its transport (i.e.) LPS binding protein (LBP) and plasma lipoproteins). LPS receptors CD14 and TLR-4 are constitutively expressed in the Central Nervous System (CNS). To our knowledge, LPS infiltration in CNS has not been clearly demonstrated. A naturalistic experiment with healthy rats was performed to investigate whether LPS is present with its receptors in brain. Immunofluorescences showed that lipid A and core LPS were present in circumventricular organs, choroid plexus, meningeal cells, astrocytes, tanycytes and endothelial cells. Co-localization of LPS regions with CD14/TLR-4 was found. The role of lipoprotein receptors (SR-BI, ApoER2 and LDLr) in the brain as targets for a LPS transport mechanism by plasma apolipoproteins (i.e. ApoAI) was studied. Co-localization of LPS regions with these lipoproteins markers was observed. Our results suggest that LPS infiltrates in the brain in physiological conditions, possibly, through a lipoprotein transport mechanism, and it is bound to its receptors in blood-brain interfaces.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-13302-6