Leptin receptor co-expression gene network moderates the effect of early life adversity on eating behavior in children

• Published in: Communications biology Vol. 5; no. 1; p. 1092
• Main Authors: de Lima, Randriely Merscher Sobreira, Barth, Barbara, Mar Arcego, Danusa, de Mendonça Filho, Euclides José, Patel, Sachin, Wang, Zihan, Pokhvisneva, Irina, Parent, Carine, Levitan, Robert D., Kobor, Michael S., de Vasconcellos Bittencourt, Ana Paula Santana, Meaney, Michael J., Dalmaz, Carla, Silveira, Patrícia Pelufo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.10.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38/43; 631/378/1488; 631/378/1831; Adverse Childhood Experiences; Biology; Biomedical and Life Sciences; Blood Glucose; Child; Eating behavior; Feeding Behavior - physiology; Food; Gene Regulatory Networks; Genes; Humans; Hypothalamus; Leptin - genetics; Leptin - metabolism; Life Sciences; Metabolic syndrome; Metabolism; Prefrontal cortex; Receptors, Leptin - genetics; Receptors, Leptin - metabolism; Satiety
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-022-03992-8

Leptin influences eating behavior. Exposure to early adversity is associated with eating behaviour disorders and metabolic syndrome, but the role of the leptin receptor on this relationship is poorly explored. We investigated whether individual differences in brain region specific leptin receptor (LepR) gene networks could moderate the effects of early adversity on eating behavior and metabolism. We created an expression-based polygenic risk score (ePRS) reflecting variations in the function of LepR gene network in prefrontal cortex and hypothalamus to investigate the interactions between a cumulative index of postnatal adversity on eating behavior in two independent birth cohorts (MAVAN and GUSTO). To explore whether variations in the prefrontal cortex or hypothalamic genetic scores could be associated with metabolic measurements, we also assessed the relationship between LepR-ePRS and fasting blood glucose and leptin levels in a third independent cohort (ALSPAC). We identified significant interaction effects between postnatal adversity and prefrontal-based LepR-ePRS on the Child Eating Behavior Questionnaire scores. In MAVAN, we observed a significant interaction effect on food enjoyment at 48 months ( β  = 61.58, p  = 0.015) and 72 months ( β  = 97.78, p  = 0.001); food responsiveness at 48 months ( β  = 83.79, p  = 0.009) satiety at 48 months ( β  = −43.63, p  = 0.047). Similar results were observed in the GUSTO cohort, with a significant interaction effect on food enjoyment ( β  = 30.48, p  = 0.006) food fussiness score ( β  = −24.07, p  = 0.02) and satiety score at 60 months ( β  = −17.00, p  = 0.037). No effects were found when focusing on the hypothalamus-based LepR-ePRS on eating behavior in MAVAN and GUSTO cohorts, and there was no effect of hypothalamus and prefrontal cortex based ePRSs on metabolic measures in ALSPAC. Our study indicated that exposure to postnatal adversity interacts with prefrontal cortex LepR-ePRS to moderate eating behavior, suggesting a neurobiological mechanism associated with the development of eating behavior problems in response to early adversity. The knowledge of these mechanisms may guide the understanding of eating patterns associated with risk for obesity in response to fluctuations in stress exposure early in life. An expression-based polygenic risk score analysis of leptin receptor (LepR) genes suggests that LepR-specific genes co-expressed in the prefrontal cortex interact with exposure to postnatal adversity, potentially modulating eating behavior in children.