Stromal-vascular fraction and adipose-derived stem cell therapies improve cartilage regeneration in osteoarthritis-induced rats

• Published in: Scientific reports Vol. 12; no. 1; pp. 2828 - 11
• Main Authors: Yang, Wan-Ting, Ke, Chun-Yen, Yeh, Kuang-Ting, Huang, Shyh-Geng, Lin, Zi-Yang, Wu, Wen-Tien, Lee, Ru-Ping
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.02.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/532; 692/420; 692/420/256; Adipocytes - transplantation; Animals; Arthritis; Cartilage; Cartilage (articular); Cartilage diseases; Cartilage, Articular - growth & development; Cartilage, Articular - pathology; Cell therapy; Cell- and Tissue-Based Therapy; Clinical trials; Disease Models, Animal; Humanities and Social Sciences; Humans; IL-1β; Inflammation; Knee; Mesenchymal Stem Cells; multidisciplinary; Osteoarthritis; Osteoarthritis - pathology; Osteoarthritis - therapy; Patients; Rats; Regeneration - genetics; Science; Science (multidisciplinary); Stem cell transplantation; Stem cells; Stromal Vascular Fraction - transplantation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06892-3

This study aimed to evaluate the effects of the stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model. Sodium iodoacetate (3 mg/50 μL) was used to induce OA in the left knee joint of rats. On day 14 after OA induction, 50 μL of SVF (5 × 10 6 cells), ADSCs (1 × 10 6  cells), or 0.9% normal saline (NS) was injected into the left knee-joint cavity of each group. The macroscopic view and histological sections revealed that the articular cartilage in the NS group was damaged, inflamed, uneven and thin, and had hyperchromatic cell infiltration. Notably, the cartilage surface had recovered to nearly normal and appeared smooth and bright on day 14 in the SVF and ADSC groups. Additionally, the white blood cell counts in the SVF and ADSC groups were higher than those in the NS group on day 14. Plasma IL-1β levels on days 7 and 14 were reduced in the SVF and ADSC groups. These results indicated that both SVF and ADSC treatments may assist in articular cartilage regeneration after cartilage injury. Cell therapy may benefit patients with OA. However, clinical trials with humans are required before the application of SVF and ADSC treatments in patients with OA.