Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions

• Published in: Scientific reports Vol. 7; no. 1; pp. 4895 - 10
• Main Authors: Guo, Yushuang, Jia, Meng-ao, Yang, Yumei, Zhan, Linlin, Cheng, Xiaofei, Cai, Jianyu, Zhang, Jie, Yang, Jie, Liu, Tao, Fu, Qiang, Zhao, Jiehong, Shamsi, Imran Haider
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.07.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 38/39; 38/43; 631/449/1659; 631/449/2661/2666; Biomarkers, Tumor - antagonists & inhibitors; Biomarkers, Tumor - genetics; Biomarkers, Tumor - immunology; Biomarkers, Tumor - metabolism; Deoxyribonucleic acid; Disease resistance; Disease Resistance - genetics; DNA; Gene expression; Gene Ontology; Gene Regulatory Networks; Genomes; Host-Pathogen Interactions; Humanities and Social Sciences; Infections; MicroRNAs - genetics; MicroRNAs - immunology; MicroRNAs - metabolism; miRNA; Molecular Sequence Annotation; multidisciplinary; Nicotiana - genetics; Nicotiana - immunology; Nicotiana - virology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - virology; Plant Proteins - genetics; Plant Proteins - immunology; Plant Proteins - metabolism; Plant resistance; Plant viruses; Potyvirus - genetics; Potyvirus - metabolism; Potyvirus - pathogenicity; RNA, Messenger - genetics; RNA, Messenger - immunology; RNA, Messenger - metabolism; RNA, Plant - genetics; RNA, Plant - immunology; RNA, Plant - metabolism; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; RNA, Viral - genetics; RNA, Viral - metabolism; Science; Science (multidisciplinary); siRNA; Tobacco; Tumor Protein, Translationally-Controlled 1
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-05155-w

Potato virus Y (PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacco in response to PVY infection. A total of 81 differentially expressed miRNAs belonging to 29 families and 8133 mRNAs were identified. The Gene Ontology (GO) enrichment analyses showed that genes encoding the DNA/RNA binding, catalytic activity and signalling molecules were all significantly enriched. Moreover, 88 miRNA-mRNA interaction pairs were identified through a combined analysis of the two datasets. We also found evidence showing that the virus-derived siRNAs (vsiRNAs) from the PVY genome target tobacco translationally controlled tumor protein ( NtTCTP ) mRNA and mediate plant resistance to PVY. Together, our findings revealed that both miRNA and mRNA expression patterns can be changed in response to PVY infection and novel vsiRNA-plant interactions that may regulate plant resistance to PVY. Both provide fresh insights into the virus-plant interactions.