Diagnosis and prognosis of myocardial infarction on a plasmonic chip

• Published in: Nature communications Vol. 11; no. 1; pp. 1654 - 9
• Main Authors: Xu, Wei, Wang, Lin, Zhang, Ru, Sun, Xuming, Huang, Lin, Su, Haiyang, Wei, Xunbin, Chen, Chia-Chun, Lou, Jiatao, Dai, Hongjie, Qian, Kun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.04.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 38/79; 59/5; 639/166/985; 639/638/542; 692/53; 692/699/75/2/1674; 82/1; 82/103; Biomarkers; Biomarkers - blood; Biomedical Engineering - methods; Biomedical materials; Calcium-binding protein; Cardiovascular diseases; Chemiluminescence; Diagnosis; Diagnostic systems; Fatalities; Fluorescence; Heart attacks; Humanities and Social Sciences; Humans; Immunoassay; Microarray Analysis - methods; Monitoring; multidisciplinary; Myocardial infarction; Myocardial Infarction - blood; Myocardial Infarction - diagnosis; Prognosis; Science; Science (multidisciplinary); Troponin; Troponin I; Troponin T - blood
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15487-3

Cardiovascular diseases lead to 31.5% of deaths globally, and particularly myocardial infarction (MI) results in 7.4 million deaths per year. Diagnosis of MI and monitoring for prognostic use are critical for clinical management and biomedical research, which require advanced tools with accuracy and speed. Herein, we developed a plasmonic gold nano-island (pGold) chip assay for diagnosis and monitoring of MI. On-chip microarray analysis of serum biomarkers (e.g., cardiac troponin I) afforded up to 130-fold enhancement of near-infrared fluorescence for ultra-sensitive and quantitative detection within controlled periods, using 10 μL of serum only. The pGold chip assay achieved MI diagnostic sensitivity of 100% and specificity of 95.54%, superior to the standard chemiluminescence immunoassay in cardiovascular clinics. Further, we monitored biomarker concentrations regarding percutaneous coronary intervention for prognostic purpose. Our work demonstrated a designed approach using plasmonic materials for enhanced diagnosis and monitoring for prognostic use towards point-of-care testing. The gold standard for diagnosis and monitoring of myocardial infarction is a chemiluminescence assay based on the detection of cardian troponin I (cTnI). Here, the authors develop a plasmonic gold nano-island chip assay for ultrasensitive detection of cTnI in as little as 10 μL of serum.