High-throughput screening of caterpillars as a platform to study host–microbe interactions and enteric immunity

• Published in: Nature communications Vol. 13; no. 1; pp. 7216 - 17
• Main Authors: Windfelder, Anton G., Müller, Frank H. H., Mc Larney, Benedict, Hentschel, Michael, Böhringer, Anna Christina, von Bredow, Christoph-Rüdiger, Leinberger, Florian H., Kampschulte, Marian, Maier, Lorenz, von Bredow, Yvette M., Flocke, Vera, Merzendorfer, Hans, Krombach, Gabriele A., Vilcinskas, Andreas, Grimm, Jan, Trenczek, Tina E., Flögel, Ulrich
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.11.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 14/28; 14/63; 45/77; 49; 59; 59/57; 59/78; 631/1647/2163; 631/250/24/2510; 631/326/2521; Animals; Antiinfectives and antibacterials; Bacteria; Colitis; Digestive system; Gastrointestinal tract; High-throughput screening; High-Throughput Screening Assays; Host Microbial Interactions; Host-Pathogen Interactions; Humanities and Social Sciences; Humans; Immunity; Inflammation; Innate immunity; Larva; Larvae; Mammals; Manduca; Manduca sexta; Medical imaging; multidisciplinary; NAD(P)H oxidase; Pathogens; Pharmacology; Phenotypes; Science; Science (multidisciplinary); Screening; Tobacco
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-34865-7

Mammalian models of human disease are expensive and subject to ethical restrictions. Here, we present an independent platform for high-throughput screening, using larvae of the tobacco hornworm Manduca sexta , combining diagnostic imaging modalities for a comprehensive characterization of aberrant phenotypes. For validation, we use bacterial/chemical-induced gut inflammation to generate a colitis-like phenotype and identify significant alterations in morphology, tissue properties, and intermediary metabolism, which aggravate with disease progression and can be rescued by antimicrobial treatment. In independent experiments, activation of the highly conserved NADPH oxidase DUOX, a key mediator of gut inflammation, leads to similar, dose-dependent alterations, which can be attenuated by pharmacological interventions. Furthermore, the developed platform could differentiate pathogens from mutualistic gastrointestinal bacteria broadening the scope of applications also to microbiomics and host-pathogen interactions. Overall, larvae-based screening can complement mammals in preclinical studies to explore innate immunity and host-pathogen interactions, thus representing a substantial contribution to improve mammalian welfare. Here, combining diagnostic imaging modalities and in vivo assays, Windfelder and colleagues established tobacco hornworm larvae Manduca sexta as an alternative high-throughput platform to study the innate immunity of the gut and host-pathogen interactions. Using the platform, the authors identify mediators of gut inflammation, differentiate pathogens from gut mutualist bacteria, and demonstrate pharmacological interventions.