From bulk, single-cell to spatial RNA sequencing

RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RN...

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Bibliographic Details
Published inInternational journal of oral science Vol. 13; no. 1; pp. 36 - 6
Main Authors Li, Xinmin, Wang, Cun-Yu
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 15.11.2021
Springer Nature B.V
Nature Publishing Group
Subjects
631/114/2785
631/208/514/1949
Dentistry
DNA sequencing
Exome Sequencing
Gene expression
Humans
Medicine
Neoplasms
Oral and Maxillofacial Surgery
Orthopedics
Precision Medicine
Review
Review Article
Ribonucleic acid
RNA
Sequence Analysis, RNA
Surgical Orthopedics
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Summary:RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.
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ISSN:1674-2818
2049-3169
2049-3169
DOI:10.1038/s41368-021-00146-0