Spatially resolved gene regulatory and disease-related vulnerability map of the adult Macaque cortex

• Published in: Nature communications Vol. 13; no. 1; p. 6747
• Main Authors: Lei, Ying, Cheng, Mengnan, Li, Zihao, Zhuang, Zhenkun, Wu, Liang, sun, Yunong, Han, Lei, Huang, Zhihao, Wang, Yuzhou, Wang, Zifei, Xu, Liqin, Yuan, Yue, Liu, Shang, Pan, Taotao, Xie, Jiarui, Liu, Chuanyu, Volpe, Giacomo, Ward, Carl, Lai, Yiwei, Xu, Jiangshan, Wang, Mingyue, Yu, Hao, Sun, Haixi, Yu, Qichao, Wang, Chunqing, Wong, Chi Wai, Liu, Wei, Xu, Liangzhi, Wei, Jingkuan, Chen, Dandan, Shang, Zhouchun, Li, Guibo, Ma, Kun, Cheng, Le, Ling, Fei, Tan, Tao, Chen, Kai, Tasic, Bosiljka, Dean, Michael, Ji, Weizhi, Yang, Huanming, Gu, Ying, Esteban, Miguel A., Li, Yuxiang, Chen, Ao, Niu, Yuyu, Zeng, Hongkui, Hou, Yong, Liu, Longqi, Liu, Shiping, Xu, Xun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.11.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 45/22; 45/23; 45/62; 45/90; 45/91; 631/378; 631/80; Accessibility; Animals; Axon guidance; Axonal transport; Brain; Calcium ions; Calcium transport; Chromatin; Chromatin - genetics; Chromatin - metabolism; Cortex (motor); Epigenetics; Female; Gene Regulatory Networks; Genes; Genetic diversity; Genetic variance; Heterogeneity; Humanities and Social Sciences; Ion transport; Macaca fascicularis - genetics; Monkeys; multidisciplinary; Neurons - metabolism; Perturbation; Prefrontal cortex; Prefrontal Cortex - metabolism; Primates; Science; Science (multidisciplinary); Topography; Transcriptomics; Visual cortex
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-34413-3

Single cell approaches have increased our knowledge about the cell type composition of the non-human primate (NHP), but a detailed characterization of area-specific regulatory features remains outstanding. We generated single-cell transcriptomic and chromatin accessibility (single-cell ATAC) data of 358,237 cells from prefrontal cortex (PFC), primary motor cortex (M1) and primary visual cortex (V1) of adult female cynomolgus monkey brain, and integrated this dataset with Stereo-seq (spatial enhanced resolution omics-sequencing) of the corresponding cortical areas to assign topographic information to molecular states. We identified area-specific chromatin accessible sites and their targeted genes, including the cell type-specific transcriptional regulatory network associated with excitatory neurons heterogeneity. We reveal calcium ion transport and axon guidance genes related to specialized functions of PFC and M1, identified the similarities and differences between adult macaque and human oligodendrocyte trajectories, and mapped the genetic variants and gene perturbations of human diseases to NHP cortical cells. This resource establishes a transcriptomic and chromatin accessibility combinatory regulatory landscape at a single-cell and spatially resolved resolution in NHP cortex. Cell type epigenetic and topographic information of primate brain is lacking. Here, authors identified transcriptional regulatory network, gradient expression pattern and disease vulnerability at cell type level in PFC, M1 and V1 of monkey brain by snRNAseq, snATAC-seq and Stereo-seq.