Single cell profiling of capillary blood enables out of clinic human immunity studies

An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing an...

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Bibliographic Details
Published inScientific reports Vol. 10; no. 1; pp. 20540 - 9
Main Authors Dobreva, Tatyana, Brown, David, Park, Jong Hwee, Thomson, Matt
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 25.11.2020
Nature Publishing Group
Nature Portfolio
Subjects
631/1647/2017
631/250/2502
Blood
Blood - metabolism
Capillaries - metabolism
Circadian Rhythm - genetics
Environmental factors
Female
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Humanities and Social Sciences
Humans
Immune system
Immunity - genetics
Male
multidisciplinary
Science
Science (multidisciplinary)
Single-Cell Analysis
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Summary:An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-77073-3