Myeloid translocation gene 16b is a dual A-kinase anchoring protein that interacts selectively with plexins in a phospho-regulated manner

• Published in: FEBS letters Vol. 584; no. 5; pp. 873 - 877
• Main Authors: Fiedler, Sarah E., Schillace, Robynn V., Daniels, Crystal J., Andrews, Sarah F., Carr, Daniel W.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.03.2010
• Subjects: A Kinase Anchor Proteins - genetics; A Kinase Anchor Proteins - metabolism; A-kinase anchoring protein; Adenosine 3′,5′-cyclic monophosphate; AKAP; Animals; cAMP; cAMP-dependent protein kinase; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cercopithecus aethiops; COS Cells; Cyclic AMP-Dependent Protein Kinase Type I - genetics; Cyclic AMP-Dependent Protein Kinase Type I - metabolism; Cyclic AMP-Dependent Protein Kinase Type II - genetics; Cyclic AMP-Dependent Protein Kinase Type II - metabolism; Drosophila; GFP; glutathione S-transferase; green fluorescent protein; GST; Humans; immunoblot; Immunoblotting; Immunoprecipitation; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; MTG; myeloid translocation gene; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Phosphoproteins - genetics; Phosphoproteins - metabolism; Phosphorylation; PKA; Plexin; Protein Binding; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; regulatory subunit of type I PKA; regulatory subunit of type II PKA; Repressor Proteins - genetics; Repressor Proteins - metabolism; RII; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; MTG; AKAP; IP; PKA; GST; cAMP; cAMP-dependent protein kinase; Adenosine 3′,5′-cyclic monophosphate; GFP; Plexin; RII; RI; IB; A-kinase anchoring protein
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2010.02.007

The myeloid translocation gene (MTG) homologue Nervy associates with PlexinA on the plasma membrane, where it functions as an A-kinase anchoring protein (AKAP) to modulate plexin-mediated semaphorin signaling in Drosophila. Mammalian MTG16b is an AKAP found in immune cells where plexin-mediated semaphorin signaling regulates immune responses. This study provides the first evidence that MTG16b is a dual AKAP capable of binding plexins. These interactions are selective (PlexinA1 and A3 bind MTG, while PlexinB1 does not) and can be regulated by PKA-phosphorylation. Collectively, these data suggest a possible mechanism for the targeting and integration of adenosine 3′,5′-cyclic monophosphate (cAMP) and semaphorin signaling in immune cells. MINT- 7556975: PlexinA3 (uniprotkb: P51805) physically interacts (MI: 0915) with MTG 16b (uniprotkb: O75081) by anti tag coimmunoprecipitation (MI: 0007) MINT- 7557008: RI alpha (uniprotkb: Q9DBC7) physically interacts (MI: 0915) with MTG 16b (uniprotkb: O75081) by anti bait coimmunoprecipitation (MI: 0006) MINT- 7556989: MTG 16b (uniprotkb: O75081) physically interacts (MI: 0915) with PlexinA3 (uniprotkb: P51805) by pull down (MI: 0096)